Shoyab M, Baluda M A
J Virol. 1975 Jan;17(1):106-113. doi: 10.1128/JVI.17.1.106-113.1976.
RNA sequence relatedness among avian RNA tumor virus genomes was analyzed by inhibition of DNA-RNA hybrid formation between 3H-labeled 35S viral RNA and an excess of leukemic or normal chicken cell DNA with increasing concentrations of unlabeled 35S viral RNA. The avian viruses tested were Rous associated virus (RAV)-3, avian myeloblastosis virus (AMV), RAV-60, RAV-61, and B-77 sarcoma virus. Hybridization of 3H-labeled 35S AMV RNA with DNA from normal chicken cells was inhibited by unlabeled 35S RAV-0 RNA as effeciently (100%) as by unlabeled AMV RNA. Hybridization between 3H-labeled 35S AMV RNA and DNA from leukemic chicken myeloblasts induced by AMV was suppressed 100 and 68% by unlabeled 35S RNA from AMV and RAV-0, respectively. Hybridization between 3H-labeled RAV-0 and leukemic chicken myeloblast DNA was inhibited 100 and 67% by unlabeled 35S RNA from RAV-0 and AMV, respectively. It appears therefore that the AMV and RAV-0 genomes are 67 to 70% homologous and that AMV hybridizes to RAV-0 like sequences in normal chicken DNA. Hybridization between AMV RNA and leukemic chicken DNA was inhibited 40% by RNA from RAV-60 or RAV-61 and 50% by B-77 RNA. Hybridization between RAV-0 RNA and leukemic chicken DNA was inhibited 80% by RAV-60 or RAV-61 and 70% by B-77 RNA. Hybridization between 3H-labeled 35S RNA from RAV-60 or RAV-61 and leukemic chicken myeloblast DNA was reduced equally by RNA from RAV-60, RAV-61, AMV or RAV-0; this suggests that RNA from RAV-60 and RAV-61 hybridizes with virus-specific sequences in leukemic DNA which are shared by AMV, RAV-0, RAV-60, and RAV-61 RNA'S. Hybridization between 3H-labeled 35S RNA from RAV-61 and normal pheasant DNA was inhibited 100% by homologous viral RNA, 22 TO 26% BY RNA from AMV or RAV-0, and 30 to 33% by RNA from RAV-60 or B-77. Nearly complete inhibition of hybricization between RAV-0 RNA and leukemic chicken DNA by a mixture of AMV and B-77 35S RNAs indicates that the RNA sequences shared by B-77 virus and RAV-0. It appears that different avian RNA tumor virus genomes have from 50 to 80% homology in nucleotide sequences and that the degree of hybridization between normal chicken cell DNA and a given viral RNA can be predicted from the homology that exists between the viral RNA tested and RAV-0 RNA.
通过用浓度递增的未标记35S病毒RNA抑制3H标记的35S病毒RNA与过量白血病或正常鸡细胞DNA之间的DNA - RNA杂交形成，分析了禽RNA肿瘤病毒基因组之间的RNA序列相关性。所测试的禽病毒有劳斯相关病毒（RAV）-3、禽成髓细胞瘤病毒（AMV）、RAV - 60、RAV - 61和B - 77肉瘤病毒。未标记的35S RAV - 0 RNA抑制3H标记的35S AMV RNA与正常鸡细胞DNA的杂交效果（100%）与未标记的AMV RNA相同。未标记的AMV和RAV - 0的35S RNA分别使3H标记的35S AMV RNA与AMV诱导的白血病鸡成髓细胞DNA之间的杂交抑制了100%和68%。未标记的RAV - 0和AMV的35S RNA分别使3H标记的RAV - 0与白血病鸡成髓细胞DNA之间的杂交抑制了100%和67%。因此，似乎AMV和RAV - 0基因组有67%至70%的同源性，并且AMV与正常鸡DNA中类似RAV - 0的序列杂交。RAV - 60或RAV - 61的RNA使AMV RNA与白血病鸡DNA之间的杂交抑制了40%，B - 77 RNA使其抑制了50%。RAV - 60或RAV - 61的RNA使RAV - 0 RNA与白血病鸡DNA之间的杂交抑制了80%，B - 77 RNA使其抑制了70%。来自RAV - 60、RAV - 61、AMV或RAV - 0的RNA使3H标记的RAV - 60或RAV - 61的35S RNA与白血病鸡成髓细胞DNA之间的杂交同等程度降低；这表明RAV - 60和RAV - 61的RNA与白血病DNA中的病毒特异性序列杂交，这些序列为AMV、RAV - 0、RAV - 60和RAV - 61的RNA所共有。同源病毒RNA使3H标记的RAV - 61的35S RNA与正常雉鸡DNA之间的杂交抑制了100%，AMV或RAV - 0的RNA使其抑制了22%至26%，RAV - 60或B - 77的RNA使其抑制了30%至33%。AMV和B - 77的35S RNA混合物几乎完全抑制了RAV - 0 RNA与白血病鸡DNA之间的杂交，这表明B - 77病毒和RAV - 0共享RNA序列。似乎不同的禽RNA肿瘤病毒基因组在核苷酸序列上有50%至80%的同源性，并且正常鸡细胞DNA与给定病毒RNA之间的杂交程度可以根据所测试的病毒RNA与RAV - 0 RNA之间存在的同源性来预测。
