Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, Rockville, MA, USA.
Am J Trop Med Hyg. 2010 Jun;82(6):1060-75. doi: 10.4269/ajtmh.2010.09-0769.
Pemphigus foliaceus is a life threatening skin disease that is associated with autoimmunity to desmoglein, a skin protein involved in the adhesion of keratinocytes. This disease is endemic in certain areas of South America, suggesting the mediation of environmental factors triggering autoimmunity. Among the possible environmental factors, exposure to bites of black flies, in particular Simulium nigrimanum has been suggested. In this work, we describe the sialotranscriptome of adult female S. nigrimanum flies. It reveals the complexity of the salivary potion of this insect, comprised by over 70 distinct genes within over 30 protein families, including several novel families, even when compared with the previously described sialotranscriptome of the autogenous black fly, S. vittatum. The uncovering of this sialotranscriptome provides a platform for testing pemphigus patient sera against recombinant salivary proteins from S. nigrimanum and for the discovery of novel pharmacologically active compounds.
落叶型天疱疮是一种危及生命的皮肤疾病，与桥粒芯糖蛋白自身免疫有关，桥粒芯糖蛋白是一种参与角蛋白细胞黏附的皮肤蛋白。这种疾病在南美的某些地区流行，提示环境因素在触发自身免疫中起介导作用。在可能的环境因素中，有人提出接触黑蝇叮咬，特别是 Simulium nigrimanum。在这项工作中，我们描述了成年雌性 S. nigrimanum 苍蝇的唾液转录组。它揭示了这种昆虫唾液成分的复杂性，由 30 多种蛋白质家族中的 70 多个不同基因组成，其中包括几个新的家族，即使与之前描述的同源黑蝇 S. vittatum 的唾液转录组相比也是如此。唾液转录组的揭示为天疱疮患者血清与来自 S. nigrimanum 的重组唾液蛋白进行检测提供了一个平台，并为发现新型具有药理活性的化合物提供了一个平台。
