Max F. Perutz Laboratories, University of Vienna, Vienna, Austria.
Cell Cycle. 2010 Jun 15;9(12):2399-402. doi: 10.4161/cc.9.12.11914.
The fission yeast Schizosaccharomyces pombe is a model organism used widely to study various aspects of eukaryotic biology. A collection of heterozygous diploid strains containing individual deletions in nearly all S. pombe genes has been created using a PCR based strategy. However, deletion of some genes has not been possible using this methodology. Here we use an efficient knockout strategy based on plasmids that contain large regions homologous to the target gene to delete an additional 29 genes. The collection of deletion mutants now covers 99% of the fission yeast open reading frames.
裂殖酵母 Schizosaccharomyces pombe 是一种广泛用于研究真核生物生物学各个方面的模式生物。使用基于 PCR 的策略创建了包含几乎所有 S. pombe 基因的单个缺失的杂合二倍体菌株的集合。然而，某些基因的缺失无法使用这种方法。在这里，我们使用一种基于含有与靶基因大片段同源的质粒的高效敲除策略来删除另外 29 个基因。现在，缺失突变体的集合涵盖了裂殖酵母开放阅读框的 99%。
