埃塞俄比亚利什曼原虫种特异性DNA探针的生成
Generation of species-specific DNA probes for Leishmania aethiopica.
作者信息
Laskay T, Kiessling R, Rinke deWit T F, Wirth D F
机构信息
Armauer Hansen Research Institute, Addis Ababa, Ethiopia.
出版信息
Mol Biochem Parasitol. 1991 Feb;44(2):279-86. doi: 10.1016/0166-6851(91)90014-w.
We report here the cloning of kinetoplast DNA (kDNA) sequences from Leishmania aethiopica in order to develop a specific and sensitive method for the identification of the parasite. Analysis of the cloned kDNA sequences showed different taxonomic specificities demonstrating sequence diversity within the kinetoplast DNA. Cloned whole minicircle hybridized with all Old World Leishmania species tested. Some cloned fragments of minicircle kDNA hybridized with Leishmania species causing cutaneous leishmaniasis in the Old World, but not with the viscerotropic species. Two L. aethiopica-specific clones were found. These clones hybridized with all L. aethiopica isolates tested, but did not react with other Leishmania species. The nucleotide sequence of the L. aethiopica-specific R3 clone is presented. Clones hybridizing with only some of the L. aethiopica isolates were also identified, although none of them showed specificity only for isolates causing localized (LCL) or diffuse (DCL) form of cutaneous leishmaniasis in Ethiopia.
我们在此报告从埃塞俄比亚利什曼原虫中克隆动基体DNA（kDNA）序列，以便开发一种用于鉴定该寄生虫的特异且灵敏的方法。对克隆的kDNA序列分析显示出不同的分类特异性，表明动基体DNA内存在序列多样性。克隆的完整微小环与所有测试的旧大陆利什曼原虫物种杂交。微小环kDNA的一些克隆片段与引起旧大陆皮肤利什曼病的利什曼原虫物种杂交，但不与内脏型物种杂交。发现了两个埃塞俄比亚利什曼原虫特异性克隆。这些克隆与所有测试的埃塞俄比亚利什曼原虫分离株杂交，但不与其他利什曼原虫物种反应。给出了埃塞俄比亚利什曼原虫特异性R3克隆的核苷酸序列。还鉴定出仅与部分埃塞俄比亚利什曼原虫分离株杂交的克隆，尽管它们中没有一个仅对在埃塞俄比亚引起局限性（LCL）或弥漫性（DCL）皮肤利什曼病的分离株具有特异性。
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