Lopes U G, Wirth D F
Mol Biochem Parasitol. 1986 Jul;20(1):77-84. doi: 10.1016/0166-6851(86)90144-1.
Several efforts have been made in order to develop more precise and sensitive methods in the identification of Leishmania parasites. We report here the identification of cloned subfragments of minicircle kinetoplast DNA (kDNA) isolated from L. donovani, WR352, which show different taxonomic specificities. Analysis of these fragments demonstrates a significant sequence diversity within the kDNA minicircle. For example, one cloned fragment was found to be present in all visceral Leishmania species tested, but was not present in any of the cutaneous Leishmania species. Another cloned fragment was only found in the strain from which it had been derived, and was not present in any of the other strains tested. In similar experiments with the New World visceral leishmanias (L. chagasi, WR518) several different cloned kDNA fragments were found to react with all of isolates of the L. chagasi tested, but not with any cutaneous Leishmania species, either from the Old World or the New World. It is of interest to note that these cloned L. chagasi kDNA fragments reacted with isolates of African visceral Leishmania species but not with isolates from India.
为了开发更精确、更灵敏的利什曼原虫鉴定方法，人们已经做出了多项努力。我们在此报告从杜氏利什曼原虫WR352分离出的微小环动基体DNA（kDNA）克隆亚片段的鉴定结果，这些片段显示出不同的分类特异性。对这些片段的分析表明，kDNA微小环内存在显著的序列多样性。例如，发现一个克隆片段存在于所有测试的内脏利什曼原虫物种中，但在任何皮肤利什曼原虫物种中均不存在。另一个克隆片段仅在所衍生的菌株中发现，在任何其他测试菌株中均不存在。在对新大陆内脏利什曼原虫（恰加斯利什曼原虫，WR518）进行的类似实验中，发现几个不同的克隆kDNA片段与所有测试的恰加斯利什曼原虫分离株发生反应，但与来自旧大陆或新大陆的任何皮肤利什曼原虫物种均无反应。值得注意的是，这些克隆的恰加斯利什曼原虫kDNA片段与非洲内脏利什曼原虫物种的分离株发生反应，但与来自印度的分离株无反应。
