Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 600 S. Mathews Avenue, Urbana, IL 61801, USA.
Chem Commun (Camb). 2010 Aug 14;46(30):5461-3. doi: 10.1039/c0cc00735h. Epub 2010 Jun 3.
We report the first example of directed evolution of a P450 monooxygenase with inverted enantioselectivity for asymmetric biohydroxylation. The biohydroxylation product of the best mutant 1AF4A has an ee of 83% (R) compared to the wild type's ee of 43% (S).
我们报道了首例通过定向进化获得 P450 单加氧酶的对映选择性反转的实例，用于不对称生物羟化反应。最佳突变体 1AF4A 的生物羟化产物的对映体过量值（ee 值）为 83%（R），而野生型的 ee 值为 43%（S）。
