Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, California 90095, USA.
J Biol Chem. 2010 Sep 3;285(36):27509-15. doi: 10.1074/jbc.R110.130419. Epub 2010 Jun 3.
Oxytetracycline (OTC) is a broad-spectrum antibiotic that acts by inhibiting protein synthesis in bacteria. It is an important member of the bacterial aromatic polyketide family, which is a structurally diverse class of natural products. OTC is synthesized by a type II polyketide synthase that generates the poly-beta-ketone backbone through successive decarboxylative condensation of malonyl-CoA extender units, followed by modifications by cyclases, oxygenases, transferases, and additional tailoring enzymes. Genetic and biochemical studies have illuminated most of the steps involved in the biosynthesis of OTC, which is detailed here as a representative case study in type II polyketide biosynthesis.
土霉素（OTC）是一种广谱抗生素，通过抑制细菌的蛋白质合成起作用。它是细菌芳香聚酮家族的重要成员，这是一类结构多样的天然产物。OTC 由 II 型聚酮合酶合成，通过丙二酰辅酶 A 延伸单元的连续脱羧缩合生成聚-β-酮骨架，然后由环化酶、加氧酶、转移酶和其他修饰酶进行修饰。遗传和生化研究阐明了 OTC 生物合成中涉及的大多数步骤，这里详细介绍了作为 II 型聚酮生物合成的代表性案例研究。
