Institut für Pharmazie, Ernst-Moritz-Arndt-Universität, Greifswald, Germany.
Appl Microbiol Biotechnol. 2010 Aug;87(6):2227-35. doi: 10.1007/s00253-010-2681-5. Epub 2010 Jun 4.
The acoABCL and acuABC operons of Bacillus licheniformis DSM13 are strongly induced at the transcriptional level during glucose starvation conditions. Primer extension analyses of this study indicate that the acoABCL operon is controlled by a sigmaL-dependent promoter and the acuABC operon by a sigmaA-dependent promoter. Transcription at the acoA promoter is repressed by glucose but induced by acetoin as soon as the preferred carbon source glucose is exhausted. The acuA promoter shows a similar induction pattern, but its activity is independent from the presence of acetoin. It is demonstrated that the acoABCL operon is mainly responsible for acetoin and 2,3-butanediol degradation in B. licheniformis.
地衣芽孢杆菌 DSM13 的 acoABCL 和 acuABC 操纵子在葡萄糖饥饿条件下，在转录水平上被强烈诱导。这项研究的引物延伸分析表明，acoABCL 操纵子由依赖 sigmaL 的启动子控制，acuABC 操纵子由依赖 sigmaA 的启动子控制。acoA 启动子的转录受葡萄糖抑制，但一旦优先碳源葡萄糖耗尽，就被乙酰基丁酮诱导。acuA 启动子显示出类似的诱导模式，但它的活性不依赖于乙酰丁酮的存在。实验证明，acoABCL 操纵子主要负责地衣芽孢杆菌中乙酰基丁酮和 2,3-丁二醇的降解。
