Xiao Fengxu, Zhang Yupeng, Zhang Lihuan, Li Siyu, Chen Wei, Shi Guiyang, Li Youran
Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China.
Microorganisms. 2024 Aug 16;12(8):1693. doi: 10.3390/microorganisms12081693.
is recognised as an exceptional expression platform in biomanufacturing due to its ability to produce high-value products. Consequently, metabolic engineering of is increasingly pursued to enhance its utility as a biomanufacturing vehicle. Effective cell factories require promoters that enable regulated expression of target genes. This review discusses recent advancements in the characterisation, synthesis, and engineering of promoters. We highlight the application of constitutive promoters, quorum sensing promoters, and inducible promoters in protein and chemical synthesis. Additionally, we summarise efforts to expand the promoter toolbox through hybrid promoter engineering, transcription factor-based inducible promoter engineering, and ribosome binding site (RBS) engineering.
由于其生产高价值产品的能力，它被公认为生物制造中一个卓越的表达平台。因此，人们越来越多地对其进行代谢工程改造，以增强其作为生物制造载体的效用。有效的细胞工厂需要能够调控目标基因表达的启动子。本综述讨论了[具体生物名称]启动子在表征、合成和工程改造方面的最新进展。我们重点介绍了组成型启动子、群体感应启动子和诱导型启动子在蛋白质和化学合成中的应用。此外，我们总结了通过杂交启动子工程、基于转录因子的诱导型启动子工程和核糖体结合位点（RBS）工程来扩展启动子工具箱的努力。
