Mansoor Tahira, Musani Mohammed Ayub, Khalid Gulnaz, Kamal Mustafa
Department of Biotechnology, University of Karachi, Karachi, Pakistan.
J Ayub Med Coll Abbottabad. 2009 Apr-Jun;21(2):120-3.
Chronic suppurative otitis media (CSOM) is a prevailing and notorious infection in developing countries causing serious local damage and threatening complications. Early and effective treatment based on the knowledge of causing micro organisms and their sensitivity results in good clinical recovery and prevents from damage and complications. Pseudomonas aeruginosa is most common pathogen causing CSOM in Pakistan. The objective of this study is to identify incidence of Pseudomonas aeruginosa involved in CSOM and sensitivity against commonly prescribed antibiotics.
A total of 263 patients with unilateral or bilateral chronic suppurative otitis media (CSOM) were enrolled in the study at Department of ENT, Karachi Medical and Dental College & Abbasi Shaheed Hospital, Karachi, Pakistan from December 2004 to May 2006. Sterile cotton swabs were used to collect pus from discharging ears and plated on Blood agar and MacConkey agar for 24 to 48 hours. Antibiotics susceptibility was tested by Minimum inhibitory concentrations (MIC) method using Mueller-Hinton agar.
Overall microbiology of 267 samples from 263 patients was studied including 4 of bilateral discharge. Polymicrobial growth was present in 8 samples. A total of 275 bacterial isolates were studied. Pseudomonas aeruginosa (40%) and Staphylococcus aureus (30.9%) were the most common bacterial agents found in CSOM. MIC was done for Pseudomonas aeruginosa only as it was the commonest pathogen found in CSOM. Sensitivity pattern of Pseudomonas aeruginosa showed that amikacin was active against 96% of isolates followed by ceftazidime 89%, ciprofloxacin 85%, gentamicin 81%, imipenem 76%, aztreonam 42% and ceftriaxone 21%.
Pseudomonas aeruginosa was the most common bacteria isolated from chronic discharging ears followed by Staphylococcus aureus. Amikacin was found to be the most suitable drug followed by ceftazidime and ciprofloxacin for Pseudomonas aeruginosa. The resistance against ceftriaxone and aztreonam was found to be very high.
慢性化脓性中耳炎（CSOM）在发展中国家是一种普遍且声名狼藉的感染性疾病，会造成严重的局部损害并引发威胁生命的并发症。基于对致病微生物及其药敏性的了解进行早期有效治疗，可实现良好的临床康复，并预防损害和并发症的发生。铜绿假单胞菌是巴基斯坦导致CSOM最常见的病原体。本研究的目的是确定参与CSOM的铜绿假单胞菌的发病率及其对常用抗生素的敏感性。
2004年12月至2006年5月期间，在巴基斯坦卡拉奇的卡拉奇医学与牙科学院及阿巴西·谢赫德医院耳鼻喉科，共有263例单侧或双侧慢性化脓性中耳炎（CSOM）患者纳入本研究。使用无菌棉签从流脓的耳朵收集脓液，并接种于血琼脂和麦康凯琼脂上培养24至48小时。采用微量肉汤稀释法，使用 Mueller-Hinton 琼脂检测抗生素敏感性。
对263例患者的267份样本进行了全面的微生物学研究，其中包括4例双侧流脓样本。8份样本存在多种微生物生长。共研究了275株细菌分离株。铜绿假单胞菌（40%）和金黄色葡萄球菌（30.9%）是CSOM中最常见的细菌病原体。仅对铜绿假单胞菌进行了 MIC 检测，因为它是CSOM中最常见的病原体。铜绿假单胞菌的药敏模式显示，阿米卡星对96%的分离株有活性，其次是头孢他啶89%、环丙沙星85%、庆大霉素81%、亚胺培南76%、氨曲南42%和头孢曲松21%。
铜绿假单胞菌是慢性流脓耳朵中分离出的最常见细菌，其次是金黄色葡萄球菌。发现阿米卡星是治疗铜绿假单胞菌最合适的药物，其次是头孢他啶和环丙沙星。发现对头孢曲松和氨曲南的耐药性非常高。
