Mechatronics and Automation Group, Suzhou Joint Research Centre of City University of Hong Kong and University of Science and Technology of China, Suzhou 215123, China.
IEEE Trans Nanobioscience. 2010 Sep;9(3):171-80. doi: 10.1109/TNB.2010.2050598. Epub 2010 Jun 3.
Microinjection has been demonstrated to be an effective technique to introduce foreign materials into biological cells. Despite the advance, whether cell injection can be used to characterize the mechanical properties of cells remains elusive. In this paper, extending the previously developed mechanical model, various constitutive materials are adopted to present the membrane characteristics of cells. To demonstrate the modeling approach and identify the most appropriate constitutive material for a specific biomembrane, finite element analysis (FEA) and experimental tests are carried out. It is shown that the modeling results agree well with those from both FEA and experiments, which demonstrates the validity of the developed approach. Moreover, Yeoh and Cheng materials are found to be the best constitutive materials in representing the deformation behaviors of zebrafish embryos and mouse embryos (or oocytes), respectively. Also, the mechanical properties of zebrafish embryos at different developmental stages and mouse embryos (or oocytes) are characterized.
显微注射已被证明是一种将外源物质引入生物细胞的有效技术。尽管有了这一进展，但细胞注射是否可以用于表征细胞的力学性能仍然难以捉摸。在本文中，通过扩展先前开发的力学模型，采用各种本构材料来呈现细胞的膜特性。为了展示建模方法并确定最适合特定生物膜的本构材料，进行了有限元分析（FEA）和实验测试。结果表明，模型结果与 FEA 和实验结果吻合较好，这证明了所开发方法的有效性。此外，发现 Yeoh 和 Cheng 材料分别是表示斑马鱼胚胎和小鼠胚胎（或卵母细胞）变形行为的最佳本构材料。还表征了不同发育阶段的斑马鱼胚胎和小鼠胚胎（或卵母细胞）的力学性能。
