Medical Mycology Research Center, Chiba University, Inohana 1-8-1, Chuo-ku, Chiba 260-8673, Japan.
Graduate School of Advanced Integration Science, Chiba University, 648 Matsudo, Matsudo, Chiba 271-8510, Japan.
Int J Syst Evol Microbiol. 2011 May;61(Pt 5):1073-1077. doi: 10.1099/ijs.0.019091-0. Epub 2010 Jun 4.
A Gram-staining-positive bacterium, designated AS-0823(T), which formed spiral spore chains on the aerial mycelium, was isolated from the intestinal tract of Armadillidium vulgare, a small terrestrial crustacean commonly found on the ground around houses in Japan. 16S rRNA gene sequence analysis showed that the isolate belonged to the genus Streptomyces and was most closely related to Streptomyces longisporus ISP 5166(T) (98.6 % 16S rRNA gene sequence similarity), Streptomyces curacoi NBRC 12761(T) (98.4 %) and Streptomyces griseoruber NBRC 12873(T) (98.4 %). The affiliation of strain AS-0823(T) to the genus Streptomyces was supported by chemotaxonomic data: iso-C(16 : 0), anteiso-C(15 : 0), C(16 : 0), iso-C(15 : 0) and anteiso-C(17 : 0) as the major cellular fatty acids, ll-diaminopimelic acid as the characteristic diamino acid in the peptidoglycan and the absence of mycolic acids. DNA-DNA hybridization and physiological and biochemical analysis supported the differentiation of strain AS-0823(T) from S. longisporus JCM 4395(T). Therefore, strain AS-0823(T) represents a novel species, for which the name Streptomyces coacervatus sp. nov. is proposed. The type strain is AS-0823(T) ( = IFM 11055(T) = DSM 41983(T) = JCM 17138(T)).
一株革兰氏阳性细菌，被命名为 AS-0823(T)，其在气生菌丝上形成螺旋孢子链，从日本房屋周围地面上常见的小型陆生甲壳动物犰狳的肠道中分离得到。16S rRNA 基因序列分析表明，该分离株属于链霉菌属，与链霉菌长孢亚种 ISP 5166(T)（98.6% 16S rRNA 基因序列相似性）、链霉菌库拉索亚种 NBRC 12761(T)（98.4%）和链霉菌灰红链霉菌 NBRC 12873(T)（98.4%）最为密切相关。菌株 AS-0823(T) 属于链霉菌属的分类学依据还包括化学分类学数据：iso-C(16:0)、anteiso-C(15:0)、C(16:0)、iso-C(15:0)和 anteiso-C(17:0) 作为主要的细胞脂肪酸，特征性二氨基庚二酸存在于肽聚糖中，并且不存在分枝菌酸。DNA-DNA 杂交和生理生化分析支持菌株 AS-0823(T) 与 S. longisporus JCM 4395(T) 的区分。因此，菌株 AS-0823(T) 代表一个新的物种，建议将其命名为 Streptomyces coacervatus sp. nov. 。模式菌株为 AS-0823(T)（=IFM 11055(T)=DSM 41983(T)=JCM 17138(T)）。
