从牛奶大罐奶中分离的星形诺卡氏菌和触酶阴性诺卡氏菌的分子鉴定和抗沸能力。
Molecular identification and thermoresistance to boiling of Nocardia farcinica and Nocardia cyriacigeorgica from bovine bulk tank milk.
机构信息
Universidade Estadual Paulista, Doenças Infecciosas de Animais Domésticos, Departamento de Higiene Veterinária e Saúde Pública, Faculdade de Medicina Veterinária , Botucatu, SP , Brasil.
出版信息
Braz J Microbiol. 2012 Jul;43(3):1038-41. doi: 10.1590/S1517-838220120003000029. Epub 2012 Jun 1.
Abstract
Two strains of Nocardia spp. were isolated from bovine milk of two individual bulk tank. Molecular identification classified the strains as Nocardia farcinica and Nocardia cyriacigeorgica. The thermorresistance to boiling of the isolates was carried out and was observed bacterial growth after boiling. Our findings indicate the potential risk of pathogen transmission to humans through contaminated milk with Nocardia spp.
摘要
从两个个体牛群贮奶罐的牛奶中分离到两株诺卡氏菌。分子鉴定将这些菌株分类为东方诺卡氏菌和触珠诺卡氏菌。对分离物的耐煮沸性进行了研究，并观察了煮沸后的细菌生长情况。我们的研究结果表明，诺卡氏菌污染的牛奶可能会对人类造成病原体传播的潜在风险。
相似文献
1
Molecular identification and thermoresistance to boiling of Nocardia farcinica and Nocardia cyriacigeorgica from bovine bulk tank milk.
Braz J Microbiol. 2012 Jul;43(3):1038-41. doi: 10.1590/S1517-838220120003000029. Epub 2012 Jun 1.
2
Molecular identification and antimicrobial susceptibility of Nocardia spp. isolated from bovine mastitis in Brazil.
Vet Microbiol. 2013 Dec 27;167(3-4):708-12. doi: 10.1016/j.vetmic.2013.08.019. Epub 2013 Sep 1.
3
Short communication: Molecular characteristics, antimicrobial susceptibility, and pathogenicity of clinical Nocardia cyriacigeorgica isolates from an outbreak of bovine mastitis.
J Dairy Sci. 2017 Oct;100(10):8414-8421. doi: 10.3168/jds.2017-12680. Epub 2017 Aug 10.
4
Molecular identification of clinical Nocardia isolates from India.
J Med Microbiol. 2015 Oct;64(10):1216-1225. doi: 10.1099/jmm.0.000143. Epub 2015 Jul 22.
5
Efficient differentiation of , and by high-resolution melting analysis using a novel locus.
J Med Microbiol. 2020 Dec;69(12):1367-1372. doi: 10.1099/jmm.0.001205. Epub 2020 May 28.
6
Identification of pathogenic Nocardia species by reverse line blot hybridization targeting the 16S rRNA and 16S-23S rRNA gene spacer regions.
J Clin Microbiol. 2010 Feb;48(2):503-11. doi: 10.1128/JCM.01761-09. Epub 2009 Dec 2.
7
Susceptibility of 186 Nocardia sp. isolates to 20 antimicrobial agents.
Antimicrob Agents Chemother. 2011 Jun;55(6):2995-8. doi: 10.1128/AAC.01279-10. Epub 2011 Mar 14.
8
from Bovine Mastitis Induced Apoptosis of Bovine Mammary Epithelial Cells via Activation of Mitochondrial-Caspase Pathway.
Front Cell Infect Microbiol. 2017 May 18;7:194. doi: 10.3389/fcimb.2017.00194. eCollection 2017.
9
Highly presumptive identification of bacterial isolates associated with the recent Canada-wide mastitis epizootic as Nocardia farcinica.
Can J Microbiol. 1993 Jul;39(7):635-41. doi: 10.1139/m93-092.
10
Identification, typing, and phylogenetic relationships of the main clinical Nocardia species in spain according to their gyrB and rpoB genes.
J Clin Microbiol. 2013 Nov;51(11):3602-8. doi: 10.1128/JCM.00515-13. Epub 2013 Aug 21.
引用本文的文献
1
First Case of Actinomycetoma in France Due to a Novel Species, sp. nov.
mSphere. 2016 Nov 23;1(6). doi: 10.1128/mSphere.00309-16. eCollection 2016 Nov-Dec.
2
Molecular identification of nocardia isolates from clinical samples and an overview of human nocardiosis in Brazil.
PLoS Negl Trop Dis. 2013 Dec 5;7(12):e2573. doi: 10.1371/journal.pntd.0002573. eCollection 2013.
本文引用的文献
1
Nocardiosis in Quebec, Canada, 1988-2008.
Clin Microbiol Infect. 2011 May;17(5):690-6. doi: 10.1111/j.1469-0691.2010.03306.x.
2
Streptomyces coacervatus sp. nov., isolated from the intestinal tract of Armadillidium vulgare.
Int J Syst Evol Microbiol. 2011 May;61(Pt 5):1073-1077. doi: 10.1099/ijs.0.019091-0. Epub 2010 Jun 4.
3
Clinical and microbiological characteristics of Nocardiosis including those caused by emerging Nocardia species in Taiwan, 1998-2008.
Clin Microbiol Infect. 2010 Jul;16(7):966-72. doi: 10.1111/j.1469-0691.2009.02950.x. Epub 2009 Oct 22.
4
Nocardiosis: an overview and additional report of 28 cases in cattle and dogs.
Rev Inst Med Trop Sao Paulo. 2008 May-Jun;50(3):177-85. doi: 10.1590/s0036-46652008005000004. Epub 2008 May 14.
5
Nocardial infections: report of 22 cases.
Rev Inst Med Trop Sao Paulo. 2007 Jul-Aug;49(4):239-46. doi: 10.1590/s0036-46652007000400009.
6
MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0.
Mol Biol Evol. 2007 Aug;24(8):1596-9. doi: 10.1093/molbev/msm092. Epub 2007 May 7.
7
An investigation of risk factors for nocardial mastitis in central Alberta dairy herds.
Can Vet J. 1991 Apr;32(4):227-31.
8
Culturing of bulk tank milk for the presence of Nocardia spp.
Can Vet J. 1990 Jun;31(6):453-4.
9
Clinical and microbiological features of nocardiosis 1997-2003.
J Med Microbiol. 2007 Apr;56(Pt 4):545-550. doi: 10.1099/jmm.0.46774-0.
10
Clinical and laboratory features of the Nocardia spp. based on current molecular taxonomy.
Clin Microbiol Rev. 2006 Apr;19(2):259-82. doi: 10.1128/CMR.19.2.259-282.2006.
Zotero 插件