Key Laboratory of Marine Biogenetic Resources, The Third Institute of Oceanography SOA, Xiamen, Fujian 361005, PR China.
Division of Integrative Environmental Sciences, Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan.
Int J Syst Evol Microbiol. 2011 Nov;61(Pt 11):2712-2716. doi: 10.1099/ijs.0.029389-0. Epub 2010 Dec 17.
The taxonomic position of an actinomycete isolated from deep-sea sediment from the Indian Ocean was determined by using a polyphasic approach. The presence of iso-C(15 : 0), anteiso-C(15 : 0), iso-C(16 : 0), iso-C(17 : 0) and anteiso-C(17 : 0) as the major cellular fatty acids, ll-diaminopimelic acid as the characteristic diamino acid, and MK-9(H(4), H(6) and H(8)) as the major menaquinones supported the affiliation of strain IH32-1(T) to the genus Streptomyces. Comparison of 16S rRNA gene sequences showed that strain IH32-1(T) exhibited highest similarities to the type strains of Streptomyces globosus (97.6 %) and Streptomyces toxytricini (97.6 %). However, DNA-DNA relatedness values between strain IH32-1(T) and the type strains of S. globosus and S. toxytricini were determined as 55.2 ± 4.7 and 38.3 ± 2.5 %, respectively. Based on its chemotaxonomic, phenotypic and genotypic characteristics, strain IH32-1(T) is considered to represent a novel species in the genus Streptomyces, for which the name Streptomyces indicus sp. nov. is proposed. The type strain is IH32-1(T) ( = DSM 42001(T) = CGMCC 4.5727(T)).
用多相分类法确定了从印度洋深海沉积物中分离出的放线菌的分类地位。存在异-C(15:0)、anteiso-C(15:0)、iso-C(16:0)、iso-C(17:0)和anteiso-C(17:0)作为主要的细胞脂肪酸、ll-二氨基庚二酸作为特征二氨基酸，以及 MK-9(H(4)、H(6)和 H(8))作为主要的menaquinones，支持菌株 IH32-1(T)属于链霉菌属。16S rRNA 基因序列比较表明，菌株 IH32-1(T)与链霉菌属的模式菌株 Streptomyces globosus(97.6%)和 Streptomyces toxytricini(97.6%)具有最高的相似性。然而，菌株 IH32-1(T)与 S. globosus 和 S. toxytricini 型菌株之间的 DNA-DNA 同源性值分别为 55.2±4.7%和 38.3±2.5%。基于其化学分类、表型和基因型特征，菌株 IH32-1(T)被认为代表链霉菌属的一个新种，命名为 Streptomyces indicus sp. nov.。其模式菌株为 IH32-1(T)(=DSM 42001(T)=CGMCC 4.5727(T))。
