Shah Ankur, Singh Harpreet, Sachdev Vibhu, Lee James, Yotsukura Sohiya, Salgia Ravi, Bharti Ajit
Department of Medicine, Section of Molecular Medicine, Boston University School of Medicine, 88 East Newton Street, Boston, MA 02118.
Curr Proteomics. 2010 Apr 1;7(1):49-65. doi: 10.2174/157016410790979635.
Lung cancer is the leading cause of cancer death for both men and women in the United States, and similar trends are seen world wide. The lack of early diagnosis is one of the primary reasons for the high mortality rate. A number of biomarkers have been evaluated in lung cancer patients, however, their specificity and early stage diagnostic values are limited. Using traditional protein chemistry and proteomics tool we have demonstrated higher serum haptoglobin levels in small cell lung cancer (SCLC). Similar findings have been reported for other cancers including ovarian cancer and glioblastoma. Haptoglobin is an acute phase protein with at least six possible phenotypes. The six phenotypes, in combination with two post translational modifications, glycosylation and deamidation, lead to large numbers of possible haptoglobin isoforms. Recent studies indicate a possible correlation between specific haptoglobin glycosylation and particular disease conditions. In our current study, we have fractionated control and SCLC patient serum by 2-D gel electrophoresis to identify differentially expressed haptoglobin isoforms in SCLC serum samples.
肺癌是美国男性和女性癌症死亡的主要原因，全球也呈现出类似趋势。缺乏早期诊断是死亡率高的主要原因之一。肺癌患者中已经评估了多种生物标志物，然而，它们的特异性和早期诊断价值有限。利用传统蛋白质化学和蛋白质组学工具，我们已经证明小细胞肺癌（SCLC）患者血清中触珠蛋白水平较高。其他癌症（包括卵巢癌和胶质母细胞瘤）也有类似的发现。触珠蛋白是一种急性期蛋白，至少有六种可能的表型。这六种表型与两种翻译后修饰（糖基化和脱酰胺化）相结合，导致大量可能的触珠蛋白异构体。最近的研究表明特定的触珠蛋白糖基化与特定疾病状况之间可能存在关联。在我们目前的研究中，我们通过二维凝胶电泳对对照和SCLC患者血清进行了分级分离，以鉴定SCLC血清样本中差异表达的触珠蛋白异构体。
