8 种希腊唇形科芳香植物精油的植物化学和杀线虫活性及 13 种萜烯成分。

Phytochemistry and nematicidal activity of the essential oils from 8 Greek Lamiaceae aromatic plants and 13 terpene components.

机构信息

Pesticide Science Laboratory, Faculty of Agriculture, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.

出版信息

J Agric Food Chem. 2010 Jul 14;58(13):7856-63. doi: 10.1021/jf100797m.

PMID:20527965

Abstract

Eight essential oils (EOs) as well as 13 single terpenes were studied for their nematicidal activity against Meloidogyne incognita , for three immersion periods (24, 48, and 96 h). The EOs were isolated from eight Greek Lamiaceae species: Melissa officinalis , Sideritis clandestina , Origanum dictamnus , Ocimum basilicum , Mentha pulegium , Origanum vulgare , Vitex agnus castus , and Salvia officinalis . The EOs nematicidal activity was correlated to their chemical composition as well as to the pure terpenes' activity tested individually. Clear dose and time response relationships were established. The EOs of O. vulgare, O. dictamnus, M. pulegium, and M. officinalis exhibited high nematicidal activity against M. incognita, and the EC(50) values (96 h) were calculated at 1.55, 1.72, 3.15, and 6.15 muL/mL, respectively. The activity of the nematicidal terpenes was found to decrease in the order l-carvone, pulegone, trans-anethole, geraniol, eugenol, carvacrol, thymol, terpinen-4-ol, and the respective EC(50) values (24 h) were calculated in the range of 115-392 mug/mL. Terpenes tested individually were more active than as components in EO, implementing antagonistic action.

摘要

研究了 8 种精油（EO）和 13 种单萜类化合物对南方根结线虫的杀线虫活性，浸泡时间为 24、48 和 96 小时。这些精油是从 8 种希腊唇形科植物中分离出来的：药用薰衣草、野生百里香、牛至、罗勒、薄荷、牛至、荆条和药用鼠尾草。EO 的杀线虫活性与其化学成分以及单独测试的纯萜类化合物的活性有关。建立了明确的剂量和时间反应关系。O. vulgare、O. dictamnus、M. pulegium 和 M. officinalis 的精油对 M. incognita 表现出高杀线虫活性，EC（50）值（96 h）分别为 1.55、1.72、3.15 和 6.15 muL/mL。杀线虫萜类化合物的活性依次为柠檬烯、黄樟素、反式茴香脑、香叶醇、丁香酚、香芹酚、百里香酚、松油醇和萜品-4-醇，各自的 EC（50）值（24 h）在 115-392 mug/mL 范围内。单独测试的萜类化合物比作为精油成分更活跃，表现出拮抗作用。