State Key Laboratory for Diagnosis and Treatment of Infectious Disease, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China.
Microb Drug Resist. 2010 Dec;16(4):297-301. doi: 10.1089/mdr.2010.0023. Epub 2010 Jun 7.
Four isolates of Klebsiella pneumoniae and one isolate of Enterobacter cloacae exhibiting resistance to most β-lactam antibiotics, including oxyimino-cephalosporins and carbapenems, were obtained from different patients among four hospitals in China. Pulsed-field gel electrophoresis demonstrated that all the K. pneumoniae isolates belonged to two clone patterns. Multilocus sequence typing showed that the four isolates of K. pneumoniae belonged to two sequence types: ST 23 and ST 351. Conjugation studies with Escherichia coli (EC600) resulted in the transfer of reduced carbapenem susceptibility compared with that of the original isolates. Plasmid restriction analysis and hybridization experiment showed that the five isolates of Enterobacteriaceae carried a common 50 kb bla(KPC-2)-encoding plasmid.
从中国四家医院的不同患者中获得了四株肺炎克雷伯菌和一株阴沟肠杆菌的分离株，这些分离株对大多数β-内酰胺抗生素（包括肟基头孢菌素和碳青霉烯类）表现出耐药性。脉冲场凝胶电泳显示，所有肺炎克雷伯菌分离株均属于两种克隆模式。多位点序列分型显示，四株肺炎克雷伯菌属于两种序列类型：ST23 和 ST351。用大肠杆菌（EC600）进行的接合研究导致与原始分离株相比，碳青霉烯类药物敏感性降低。质粒限制分析和杂交实验表明，五株肠杆菌科细菌携带一个共同的 50kbbla(KPC-2)-编码质粒。
