School of Medicine, Deakin University, 75 Pigdons Road, Waurn Ponds, Victoria 3216, Australia.
Centre for Molecular and Medical Research, Deakin University, 75 Pigdons Road, Waurn Ponds, Victoria 3216, Australia.
Int J Mol Sci. 2020 Apr 3;21(7):2485. doi: 10.3390/ijms21072485.
This planet is home to countless species, some more well-known than the others. While we have developed many techniques to be able to interrogate some of the "omics", proteomics is becoming recognized as a very important part of the puzzle, given how important the protein is as a functional part of the cell. Within human health, the proteome is fairly well-established, with numerous reagents being available to decipher cellular pathways. Recent research advancements have assisted in characterizing the proteomes of some model (non-human) species, however, in many other species, we are only just touching the surface. This review considers three main reagent classes-antibodies, aptamers, and nanobodies-as a means of continuing to investigate the proteomes of non-model species without the complications of understanding the full protein signature of a species. Considerations of ease of production, potential applications, and the necessity for producing a new reagent depending on homology are presented.
这个星球是无数物种的家园，有些比其他的更出名。虽然我们已经开发了许多技术来能够询问一些“组学”，蛋白质组学正被认为是拼图中非常重要的一部分，因为蛋白质作为细胞的功能部分是多么重要。在人类健康中，蛋白质组学已经相当成熟，有许多试剂可用于破译细胞途径。最近的研究进展有助于描述一些模式（非人类）物种的蛋白质组，但在许多其他物种中，我们才刚刚开始触及表面。这篇综述考虑了三种主要的试剂类别——抗体、适体和纳米体——作为一种继续研究非模式物种蛋白质组的方法，而不必了解一个物种的完整蛋白质特征。本文介绍了生产的难易程度、潜在应用以及根据同源性生产新试剂的必要性等方面的考虑。
