零售小麦粉中霉菌区系及黄曲霉毒素污染的分子特征。
Molecular characterization of mycobiota and aflatoxin contamination of retail wheat flours from Jeddah markets.
机构信息
Girls College, King Abdul Aziz University, Jeddah, Saudi Arabia.
出版信息
Foodborne Pathog Dis. 2010 Sep;7(9):1047-54. doi: 10.1089/fpd.2009.0506.
The mycological profile of the retail wheat flour selling in different markets at Jeddah (Kingdom of Saudi Arabia) was studied. The most common genera were Aspergillus (isolated from 70% of the tested samples), Penicillium (30%), Eurotium (14%), and in a lesser extent Fusarium (20%) and Alternaria (18%). Twenty-nine strains of Aspergillus flavus were screened for their ability to produce aflatoxins (AFs). Four strains produced only aflatoxin B1 (AFB1), two strains produced AFB1 and aflatoxin B2, and one strain produced AFB1, aflatoxin G1, and aflatoxin G2. Random amplified polymorphic DNA-polymerase chain reaction technique could not differentiate between toxigenic and nontoxigenic strains of A. flavus. AF regulatory gene was detected in three flour samples and in seven A. flavus isolates.
对吉达（沙特阿拉伯王国）不同市场销售的零售小麦粉的真菌区系进行了研究。最常见的属是曲霉属（从 70%的测试样本中分离到）、青霉属（30%）、帚霉属（14%），在较小程度上还有镰刀菌属（20%）和交链孢属（18%）。从 29 株黄曲霉中筛选出了产黄曲霉毒素（AFs）的能力。有 4 株只产生黄曲霉毒素 B1（AFB1），有 2 株产生 AFB1 和黄曲霉毒素 B2，有 1 株产生 AFB1、黄曲霉毒素 G1 和黄曲霉毒素 G2。随机扩增多态性 DNA-聚合酶链反应技术无法区分产毒和非产毒黄曲霉菌株。在 3 个面粉样品和 7 个黄曲霉分离物中检测到了 AF 调节基因。
Zotero 插件