Department of Molecular Health Sciences, Nagoya City University, Japan.
FEMS Microbiol Lett. 2010 May;306(2):103-9. doi: 10.1111/j.1574-6968.2010.01947.x.
In order to evaluate the biochemical characteristics of 14 substrains of Mycobacterium bovis bacillus Calmette Guérin (BCG) - Russia, Moreau, Japan, Sweden, Birkhaug, Danish, Glaxo, Mexico, Tice, Connaught, Montreal, Phipps, Australia and Pasteur - we performed eight different biochemical tests, including those for nitrate reduction, catalase, niacin accumulation, urease, Tween 80 hydrolysis, pyrazinamidase, p-amino salicylate degradation and resistance to thiophene 2-carboxylic acid hydrazide. Catalase activities of the substrains were all low. Data for nitrate reduction, niacin accumulation, Tween 80 hydrolysis, susceptibility to hydrogen peroxide and nitrate, and optimal pH for growth were all variable among these substrains. These findings suggest that the heterogeneities of biochemical characteristics are relevant to the differences in resistance of BCG substrains to environmental stress. The study also contributes to the re-evaluation of BCG substrains for use as vaccines.
为了评估 14 株牛分枝杆菌卡介苗（BCG）的俄罗斯、莫罗、日本、瑞典、伯克霍格、丹麦、葛兰素史克、墨西哥、泰西、康诺特、蒙特利尔、菲普斯、澳大利亚和巴斯德亚系的生化特性，我们进行了八项不同的生化试验，包括硝酸盐还原、过氧化氢酶、烟酸积累、脲酶、吐温 80 水解、吡嗪酰胺酶、对氨基水杨酸降解和对噻吩 2-羧酸肼的抗性。这些亚系的过氧化氢酶活性均较低。硝酸盐还原、烟酸积累、吐温 80 水解、对过氧化氢和硝酸盐的敏感性以及最佳生长 pH 值的数据在这些亚系之间均存在差异。这些发现表明，生化特性的异质性与 BCG 亚系对环境应激的抗性差异有关。该研究还有助于重新评估 BCG 亚系作为疫苗的应用。
