Sanchini Andrea, Dematheis Flavia, Semmler Torsten, Lewin Astrid
Division 16, Mycotic and Parasitic Agents and Mycobacteria, Robert Koch Institute , Berlin , Germany.
Institute of Microbiology and Epizootics, Free University Berlin , Berlin , Germany.
PeerJ. 2017 Jan 3;5:e2833. doi: 10.7717/peerj.2833. eCollection 2017.
subsp. (MAH) is an emerging opportunistic human pathogen. It can cause pulmonary infections, lymphadenitis and disseminated infections in immuno-compromised patients. In addition, MAH is widespread in the environment, since it has been isolated from water, soil or dust. In recent years, knowledge on MAH at the molecular level has increased substantially. In contrast, knowledge of the MAH metabolic phenotypes remains limited.
In this study, for the first time we analyzed the metabolic substrate utilization of ten MAH isolates, five from a clinical source and five from an environmental source. We used BIOLOG Phenotype Microarray technology for the analysis. This technology permits the rapid and global analysis of metabolic phenotypes.
The ten MAH isolates tested showed different metabolic patterns pointing to high intra-species diversity. Our MAH isolates preferred to use fatty acids such as Tween, caproic, butyric and propionic acid as a carbon source, and L-cysteine as a nitrogen source. Environmental MAH isolates resulted in being more metabolically active than clinical isolates, since the former metabolized more strongly butyric acid ( = 0.0209) and propionic acid ( = 0.00307).
Our study provides new insight into the metabolism of MAH. Understanding how bacteria utilize substrates during infection might help the developing of strategies to fight such infections.
亚种（MAH）是一种新出现的机会性人类病原体。它可在免疫功能低下的患者中引起肺部感染、淋巴结炎和播散性感染。此外，MAH在环境中广泛存在，因为它已从水、土壤或灰尘中分离出来。近年来，在分子水平上对MAH的了解有了大幅增加。相比之下，对MAH代谢表型的了解仍然有限。
在本研究中，我们首次分析了10株MAH分离株的代谢底物利用情况，其中5株来自临床来源，5株来自环境来源。我们使用BIOLOG表型微阵列技术进行分析。该技术允许对代谢表型进行快速和全面的分析。
所测试的10株MAH分离株显示出不同的代谢模式，表明种内多样性较高。我们的MAH分离株更喜欢使用吐温、己酸、丁酸和丙酸等脂肪酸作为碳源，以及L-半胱氨酸作为氮源。环境来源的MAH分离株在代谢上比临床分离株更活跃，因为前者对丁酸（P = 0.0209）和丙酸（P = 0.00307）的代谢更强。
我们的研究为MAH的代谢提供了新的见解。了解细菌在感染过程中如何利用底物可能有助于制定对抗此类感染的策略。
