Buckley P J
Department of Pathology, Yale University School of Medicine, New Haven, CT 065110.
Scanning Microsc. 1991 Mar;5(1):147-57; discussion 157-8.
Using immunohistochemical techniques and a large number of monoclonal antibodies, the presence and distribution of phenotypic subpopulations of macrophages (MOs) and dendritic cells in human spleen were assessed. The results of this study show that different subsets of MOs and dendritic cells are present in the spleen and that some of these occupy discrete microanatomic locations. In the red pulp (RP) certain antigens are expressed by different proportions of uniformly distributed MOs in the cords. On the other hand, some antigens are present on MOs that form clusters of variable size within the red pulp. These include CD11c, CD15 and alpha-1-anti-chymotrypsin. Another type of cell in the RP that is phagocytic under certain conditions is the splenic sinusoidal lining cell (SLC). These cells exhibit a phenotype that is unique: nonspecific esterase (NSE)+, lysozyme+, and HLA-DR+, CD36+, factor VIII-related antigen+, CD8+ and CD71+. MOs in the splenic marginal zone (MZ) share some antigens with red pulp MOs, but in addition express CD11b, CD14 (Mo2;63D3) and 61D3. These antigens are found on only a few RP MOs. MZ cells expressing one antigen shared with RP MOs (CD4) and one present largely on the MZ cells (CD14;63D3) form clusters around small vessels; these structures resemble the so-called splenic ellipsoids that may play a role in the trapping of circulating antigens. Phagocytic MOs (tangible body MOs) of the white pulp follicular germinal centers were also shown to differ from RP and MZ cells with respect to the expression of the antigens CD11b, CD14 (Leu M3;Mo2), CD16 and the antigen detected by antibody 25F9. The unique topographical and surface antigenic features of dendritic cells were confirmed by this study. Furthermore, these cells were found to share a number of antigens with RP, MZ, and white pulp MOs, which suggests that they may be derived from a common progenitor. The presence of phenotypic subpopulations and variation in distribution among human splenic phagocytic cells and dendritic cells may be indicative of functional specialization.
运用免疫组织化学技术和大量单克隆抗体，对人脾脏中巨噬细胞（MOs）和树突状细胞表型亚群的存在及分布进行了评估。本研究结果表明，脾脏中存在不同亚群的MOs和树突状细胞，其中一些占据离散的微观解剖位置。在红髓（RP）中，某些抗原由红髓索中均匀分布的不同比例的MOs表达。另一方面，一些抗原存在于在红髓内形成大小不一的簇的MOs上。这些抗原包括CD11c、CD15和α-1-抗糜蛋白酶。红髓中另一种在特定条件下具有吞噬作用的细胞是脾窦衬里细胞（SLC）。这些细胞表现出独特的表型：非特异性酯酶（NSE）阳性、溶菌酶阳性、HLA-DR阳性、CD36阳性、因子VIII相关抗原阳性、CD8阳性和CD71阳性。脾边缘区（MZ）的MOs与红髓MOs有一些共同抗原，但还表达CD11b、CD14（Mo2；63D3）和61D3。这些抗原仅在少数红髓MOs上发现。表达一种与红髓MOs共有的抗原（CD4）和一种主要存在于边缘区细胞上的抗原（CD14；63D3）的边缘区细胞在小血管周围形成簇；这些结构类似于所谓的脾椭球体，可能在循环抗原的捕获中起作用。白髓滤泡生发中心的吞噬性MOs（含铁血黄素巨噬细胞）在抗原CD11b、CD14（Leu M3；Mo2）、CD16和抗体25F9检测到的抗原表达方面也与红髓和边缘区细胞不同。本研究证实了树突状细胞独特的拓扑和表面抗原特征。此外，发现这些细胞与红髓、边缘区和白髓MOs有许多共同抗原，这表明它们可能来自共同的祖细胞。人脾吞噬细胞和树突状细胞中表型亚群的存在及分布差异可能表明功能特化。
