Buckley P J, Smith M R, Braverman M F, Dickson S A
Am J Pathol. 1987 Sep;128(3):505-20.
Macrophages (M phi s) are an important component of the immune response and mediate numerous other functions. Phenotypic and functional subsets of circulating monocytes have been described, but few similar studies have analyzed M phi s in human tissues. By use of immunohistochemical techniques and a large number of monoclonal antibodies, the presence and distribution of phenotypic subpopulations of M phi s and dendritic cells in human spleen were assessed. The results of this study show that different subsets of M phi s and dendritic cells are present in the spleen and that some of these occupy discrete microanatomic locations. In the red pulp (RP) certain groups of antigens are expressed by different proportions of uniformly distributed M phi s in the cords. On the other hand, some antigens are present on M phi s that form clusters of variable size within the red pulp. M phi s in the splenic marginal zone (MZ) share some antigens with red pulp M phi s, but in addition express CR3, Mo-2, 61D3, and 63D3. These antigens are found on only a few RP M phi s. MZ cells expressing one antigen shared with RP M phi s (Leu-3a,b) and one present largely on the MZ cells (63D3) form clusters around small vessels; these structures resemble the so-called splenic ellipsoids that may play a role in the trapping of circulating antigens. Phagocytic M phi s (tingible body M phi s) of the white pulp follicular germinal centers were also shown to differ from RP and MZ cels with respect to the expression of the antigens detected by anti-FcR, Leu-M3, Mo-2, 25F9, and anti-CR3. The unique topographical and surface antigenic features of dendritic cells were confirmed by this study. Furthermore, these cells were found to share a number of antigens with RP, MZ, and white pulp M phi s, which suggests that they may be derived from a common progenitor. The presence of phenotypic subpopulations and variation in distribution among human splenic phagocytic cells and dendritic cells may be indicative of functional specialization.
巨噬细胞是免疫反应的重要组成部分，并介导许多其他功能。循环单核细胞的表型和功能亚群已被描述，但很少有类似研究分析人体组织中的巨噬细胞。通过使用免疫组织化学技术和大量单克隆抗体，评估了人脾脏中巨噬细胞和树突状细胞表型亚群的存在和分布。本研究结果表明，脾脏中存在不同亚群的巨噬细胞和树突状细胞，其中一些占据离散的微观解剖位置。在红髓中，某些抗原组由红髓索中均匀分布的不同比例的巨噬细胞表达。另一方面，一些抗原存在于在红髓内形成大小可变的簇的巨噬细胞上。脾边缘区的巨噬细胞与红髓巨噬细胞共享一些抗原，但此外还表达CR3、Mo-2、61D3和63D3。这些抗原仅在少数红髓巨噬细胞上发现。表达一种与红髓巨噬细胞共享的抗原（Leu-3a、b）和一种主要存在于边缘区细胞上的抗原（63D3）的边缘区细胞在小血管周围形成簇；这些结构类似于所谓的脾椭球体，可能在循环抗原捕获中起作用。白髓滤泡生发中心的吞噬性巨噬细胞（含铁血黄素巨噬细胞）在抗FcR、Leu-M3、Mo-2、25F9和抗CR3检测的抗原表达方面也与红髓和边缘区细胞不同。本研究证实了树突状细胞独特的地形学和表面抗原特征。此外，发现这些细胞与红髓、边缘区和白髓巨噬细胞共享许多抗原，这表明它们可能来自共同的祖细胞。人脾吞噬细胞和树突状细胞中表型亚群的存在和分布差异可能表明功能特化。
