Department of Gynecology and Obstetrics, Botucatu Medical School São Paulo State University-UNESP, Botucatu, Brazil.
Reprod Biol Endocrinol. 2010 Jun 7;8:56. doi: 10.1186/1477-7827-8-56.
Although the motile sperm organelle morphology examination (MSOME) was developed only as a selection criterion, its application as a method for classifying sperm morphology may represent an improvement in evaluation of semen quality, with potential clinical repercussions. The present study aimed to evaluate individual variations in the motile sperm organelle morphology examination (MSOME) analysis after a time interval.
Two semen samples were obtained from 240 men from an unselected group of couples undergoing infertility investigation and treatment. Mean time interval between the two semen evaluations was 119+/-102 days. No clinical or surgical treatment was realized between the two observations. Spermatozoa were analyzed at greater than or equal to 8400x magnification by inverted microscope equipped with DIC/Nomarski differential interference contrast optics. At least 200 motile spermatozoa per semen sample were evaluated and percentages of normal spermatozoa and spermatozoa with large nuclear vacuoles (LNV/one or more vacuoles occupying>50% of the sperm nuclear area) were determined. A spermatozoon was classified as morphologically normal when it exhibited a normal nucleus (smooth, symmetric and oval nucleus, width 3.28+/-0.20 microm, length 4.75+/-0.20 microm/absence of vacuoles occupying>4% of nuclear area) as well as acrosome, post-acrosomal lamina, neck and tail, besides not presenting cytoplasm around the head. One examiner, blinded to subject identity, performed the entire study.
Mean percentages of morphologically normal and LNV spermatozoa were identical in the two MSOME analyses (1.6+/-2.2% vs. 1.6+/-2.1% P=0.83 and 25.2+/-19.2% vs. 26.1+/-19.0% P=0.31, respectively). Regression analysis between the two samples revealed significant positive correlation for morphologically normal and for LNV spermatozoa (r=0.57 95% CI:0.47-0.65 P<0.0001 and r=0.50 95% CI:0.38-0.58 P<0.0001, respectively).
The significant positive correlation and absence of differences between two sperm samples evaluated after a time interval with respect to normal morphology and LNV spermatozoa indicated that MSOME seems reliable (at least for these two specific sperm forms) for analyzing semen. The present result supports the future use of MSOME as a routine method for semen analysis.
虽然运动精子细胞器形态检查（MSOME）仅作为选择标准而开发，但将其应用于精子形态分类可能会改善精液质量评估，并具有潜在的临床意义。本研究旨在评估两次精液评估之间 MSOME 分析的个体变化。
从 240 名接受不孕调查和治疗的夫妇中未选择的一组男性中获得两份精液样本。两次精液评估之间的平均时间间隔为 119+/-102 天。两次观察之间未进行任何临床或手术治疗。使用配备 DIC/Nomarski 微分干涉对比光学装置的倒置显微镜以大于或等于 8400x 的放大倍数分析精子。每份精液样本评估至少 200 个活动精子，确定正常精子和具有大核空泡的精子（一个或多个空泡占据核面积>50%）的百分比。当精子显示正常核（光滑、对称和椭圆形核，宽度 3.28+/-0.20 微米，长度 4.75+/-0.20 微米/无空泡占据核面积>4%）以及顶体、顶体后板、颈部和尾部，并且头部周围没有细胞质时，将精子归类为形态正常。一位检查者对受检者的身份进行了盲法评估。
两次 MSOME 分析中形态正常和 LNV 精子的百分比相同（分别为 1.6+/-2.2%与 1.6+/-2.1%，P=0.83 和 25.2+/-19.2%与 26.1+/-19.0%，P=0.31）。两个样本之间的回归分析显示形态正常和 LNV 精子呈显著正相关（r=0.57，95%置信区间：0.47-0.65，P<0.0001 和 r=0.50，95%置信区间：0.38-0.58，P<0.0001）。
两次精液样本在时间间隔后评估时，正常形态和 LNV 精子的显著正相关且无差异表明 MSOME 似乎是可靠的（至少对于这两种特定的精子形式），适用于精液分析。本研究结果支持将 MSOME 作为常规的精液分析方法。
