Oliveira J B A, Massaro F C, Mauri A L, Petersen C G, Nicoletti A P M, Baruffi R L R, Franco J G
Centre for Human Reproduction Professor Franco Jr, Ribeirão Preto, São Paulo, Brazil.
Reprod Biomed Online. 2009 Mar;18(3):320-6. doi: 10.1016/s1472-6483(10)60088-0.
The present study aimed to evaluate the correlation between the motile sperm organelle morphology examination (MSOME) and a well-known sperm morphology classification (Tygerberg criteria). For MSOME, spermatozoa were analysed at x8400 magnification by inverted microscope equipped with Nomarski differential interference contrast optics, Uplan Apo x100 oil/1.35 objective lens and variable zoom lens. By Tygerberg criteria, the semen underwent morphological evaluation as described in the literature. Regression analysis demonstrated significant positive correlation between percentage of normal sperm forms by Tygerberg criteria and by MSOME (r = 0.83, P < 0.0001). However, the incidence of normal spermatozoa by Tygerberg criteria (9.4%) was significantly higher (P < 0.0001) than under MSOME (3.3%). Despite the highly positive correlation, MSOME is a much stricter criterion of sperm morphology classification, since it identifies vacuoles and chromatin abnormalities that are not evaluated with the same precision by the analysis of Tygerberg criteria. MSOME should be included among the routine criteria for semen analysis. In addition, MSOME should be used for selection of spermatozoa for intracytoplasmic sperm injection based on the already published literature, as this is a good selection tool.
本研究旨在评估活动精子细胞器形态检查（MSOME）与一种广为人知的精子形态分类方法（泰格堡标准）之间的相关性。对于MSOME，使用配备了诺马斯基微分干涉差光学系统、Uplan Apo x100油浸/1.35物镜和可变变焦镜头的倒置显微镜，在8400倍放大倍数下对精子进行分析。按照泰格堡标准，精液按照文献中所述进行形态学评估。回归分析表明，泰格堡标准下和MSOME下正常精子形态的百分比之间存在显著正相关（r = 0.83，P < 0.0001）。然而，泰格堡标准下正常精子的发生率（9.4%）显著高于MSOME下的发生率（3.3%）（P < 0.0001）。尽管存在高度正相关，但MSOME是一种更为严格的精子形态分类标准，因为它能够识别液泡和染色质异常，而泰格堡标准分析无法以相同的精度对这些异常进行评估。MSOME应纳入精液分析的常规标准之中。此外，根据已发表的文献，MSOME应用于胞浆内单精子注射的精子选择，因为这是一种很好的选择工具。
