Section of Clinical Pathology and Unit for Human Reproduction Pathology, Department of Medicine, University of Padova, Padova, Italy.
Institute of Gynecology and Obstetrics, University of Padova, Padova, Italy.
Fertil Steril. 2014 Mar;101(3):647-52. doi: 10.1016/j.fertnstert.2013.11.029. Epub 2013 Dec 20.
To evaluate DNA fragmentation in single sperm selected by both birefringence and motile sperm organelle morphology examination (MSOME) with a single instrument.
Prospective study.
University setting.
PATIENT(S): Semen samples from 33 normozoospermic subjects.
INTERVENTION(S): Birefringence and MSOME to distinguish different categories of sperm: nonbirefringent (category A), birefringent (category B), birefringent with nuclear vacuoles (category C), and birefringent with no nuclear vacuoles (category D). From each semen sample, sperm of any category were selected and further analyzed by TUNEL test.
MAIN OUTCOME MEASURE(S): A total of 660 well-characterized sperm were evaluated for DNA fragmentation.
RESULT(S): Category A showed a low percentage of sperm with normal MSOME results (19.4%) and high prevalence of DNA fragmentation (70.3%). Category B had 81.8% normal MSOME results, and in this group 31.8% had fragmentated DNA. Category C showed 31.8% and 92.6% DNA fragmentation in sperm with small and large nuclear vacuoles, respectively. Birefringent sperm with normal MSOME results and no vacuoles showed the lowest percentage of fragmented DNA (2.8%).
CONCLUSION(S): Sperm selection by birefringence or MSOME alone had one-third probability to select sperm with fragmented DNA. The lowest percentage of DNA fragmentation was found in birefringent sperm with no nuclear vacuoles and normal MSOME results. We suggest combining both methods using a single microscope and selecting sperm without nuclear vacuoles to get sperm with a higher chance of having intact DNA.
使用单一仪器评估通过双折射和运动精子细胞器形态检查（MSOME）选择的单个精子的 DNA 碎片化。
前瞻性研究。
大学环境。
33 名正常精子症患者的精液样本。
双折射和 MSOME 区分不同类别的精子：非双折射（A 类）、双折射（B 类）、双折射伴核空泡（C 类）和双折射伴无核空泡（D 类）。从每个精液样本中，选择任何类别的精子，并通过 TUNEL 测试进一步分析。
总共评估了 660 个特征良好的精子的 DNA 碎片化情况。
A 类显示具有正常 MSOME 结果的精子比例较低（19.4%），而 DNA 碎片化的患病率较高（70.3%）。B 类有 81.8%的精子具有正常的 MSOME 结果，其中 31.8%的精子 DNA 碎片化。C 类显示具有小核空泡和大核空泡的精子的 DNA 碎片化分别为 31.8%和 92.6%。具有正常 MSOME 结果和无空泡的双折射精子显示出最低的 DNA 碎片化比例（2.8%）。
单独通过双折射或 MSOME 选择精子，有三分之一的可能性选择具有 DNA 碎片化的精子。在具有正常 MSOME 结果和无核空泡的双折射精子中发现的 DNA 碎片化比例最低。我们建议使用单一显微镜结合这两种方法，并选择无核空泡的精子，以获得具有完整 DNA 的精子的机会更高。
