Department of Oral and Maxillofacial Surgery, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe, Hyogo 650-0017, Japan.
J Dent Res. 2010 Aug;89(8):854-9. doi: 10.1177/0022034510370022. Epub 2010 Jun 8.
Bone marrow stromal cells (BMSCs)/beta-tricalcium phosphate (beta-TCP) composites have attracted a great deal of attention in bone tissue engineering. If more effective bone regeneration is to be achieved, efficient cell-seeding systems need to be clarified. In this study, we investigated the number of cells contained in composites, and the in vitro/vivo osteogenic differentiation capacity of composites using 4 conventional systems of seeding rat BMSCs into beta-TCP: soak, low-pressure, pipette, and syringe systems. The highest number of cells was contained in the composites from the syringe group. Moreover, after two-week osteogenic induction in vitro, the composites in the syringe group exhibited the highest osteogenic potential, which continued at 8 weeks after subcutaneous implantation in vivo. Our results indicated that efficient and appropriate cell-seeding could improve in vitro/vivo bone formation in composites and thus make a potential clinical contribution to successful bone tissue engineering.
骨髓基质细胞（BMSCs）/β-磷酸三钙（β-TCP）复合材料在骨组织工程中受到了广泛关注。如果要实现更有效的骨再生，就需要阐明有效的细胞接种系统。在这项研究中，我们使用 4 种常规的将大鼠 BMSCs 接种到β-TCP 中的方法（浸泡、低压、吸管和注射器），研究了复合材料中包含的细胞数量和复合材料的体外/体内成骨分化能力。注射器组的复合材料中包含的细胞数量最多。此外，在体外进行两周的成骨诱导后，注射器组的复合材料表现出最高的成骨潜力，这种潜力在体内皮下植入 8 周后仍在持续。我们的结果表明，有效的细胞接种可以改善复合材料的体外/体内成骨能力，从而为成功的骨组织工程做出潜在的临床贡献。
