Mustahsan Vamiq M, Anugu Amith, Komatsu David E, Kao Imin, Pentyala Srinivas
Department of Anesthesiology, Stony Brook University, Stony Brook, NY 11794, USA.
Department of Mechanical Engineering, Stony Brook University, Stony Brook, NY 11794, USA.
Bioengineering (Basel). 2021 Nov 30;8(12):199. doi: 10.3390/bioengineering8120199.
Currently used synthetic bone graft substitutes (BGS) are either too weak to bear the principal load or if metallic, they can support loading, but can lead to stress shielding and are unable to integrate fully. In this study, we developed biocompatible, 3D printed scaffolds derived from µCT images of the bone that can overcome these issues and support the growth of osteoblasts.
Cylindrical scaffolds were fabricated with acrylonitrile butadiene styrene (ABS) and Stratasys MED 610 (MED610) materials. The 3D-printed scaffolds were seeded with calvaria cells (MC3T3). After the cells attained confluence, osteogenesis was induced with and without the addition of calcitonin receptor fragment peptide (CRFP) and the bone matrix production was analyzed. Mechanical compression testing was carried out to measure compressive strength, stiffness, and elastic modulus.
For the ABS scaffolds, there was a 9.8% increase in compressive strength ( < 0.05) in the scaffolds with no pre-coating and the treatment with CRFP, compared to non-treated scaffolds. Similarly, MED610 scaffolds treated with CRFP showed an 11.9% (polylysine pre-coating) and a 20% (no pre-coating) increase ( < 0.01) in compressive strength compared to non-treated scaffolds.
MED610 scaffolds are excellent BGS as they support osteoblast growth and show enhanced bone growth with enhanced compressive strength when augmented with CRFP.
目前使用的合成骨移植替代物（BGS）要么强度太弱无法承受主要负荷，要么如果是金属材质，虽能支撑负荷，但会导致应力遮挡且无法完全整合。在本研究中，我们从骨骼的µCT图像中开发出了生物相容性的3D打印支架，可克服这些问题并支持成骨细胞生长。
用丙烯腈丁二烯苯乙烯（ABS）和Stratasys MED 610（MED610）材料制作圆柱形支架。将颅盖细胞（MC3T3）接种到3D打印的支架上。细胞汇合后，在添加和不添加降钙素受体片段肽（CRFP）的情况下诱导成骨，并分析骨基质生成情况。进行机械压缩测试以测量抗压强度、刚度和弹性模量。
对于ABS支架，与未处理的支架相比，未预涂层且用CRFP处理的支架抗压强度增加了9.8%（P<0.05）。同样，与未处理的支架相比，用CRFP处理的MED610支架抗压强度增加了11.9%（聚赖氨酸预涂层）和20%（未预涂层）（P<0.01）。
MED610支架是优秀的骨移植替代物，因为它们支持成骨细胞生长，并且在用CRFP增强后，抗压强度增强，骨生长也得到增强。
