溴莫尼定对人视网膜色素上皮细胞系跨上皮电阻的影响。
The effect of brimonidine on transepithelial resistance in a human retinal pigment epithelial cell line.
作者信息
Park Jung Hyun, Kim Sung Joon, Yu Hyeong Gon
机构信息
Department of Ophthalmology, Seoul Paik Hospital, Inje University College of Medicine, Seoul, Korea.
出版信息
Korean J Ophthalmol. 2010 Jun;24(3):169-72. doi: 10.3341/kjo.2010.24.3.169. Epub 2010 Jun 5.
PURPOSE
To investigate the effects of brimonidine, an alpha-2-adrenergic agonist, on barrier function in ARPE-19 cells by measuring transepithelial resistance (TER).
METHODS
ARPE-19 cells were cultured into a confluent monolayer on a microporous filter. Brimonidine was added to the apical medium, and the barrier function of the cells was evaluated by measuring TER. A subset of cells was treated under hypoxic conditions, and the TER changes observed upon administration of brimonidine were compared to those observed in cells in normoxic conditions.
RESULTS
The ARPE cell membrane reached a peak resistance of 29.1+/-7.97 Omegacm(2) after four weeks of culture. The TER of the cells treated under normoxic conditions increased with brimonidine treatment; however, the TER of the cells treated under hypoxic conditions did not change following the administration of brimonidine.
CONCLUSIONS
Barrier function in ARPE-19 cells increased with brimonidine treatment. Understanding the exact mechanism of this barrier function change requires further investigation.
目的
通过测量跨上皮电阻（TER），研究α2肾上腺素能激动剂溴莫尼定对ARPE - 19细胞屏障功能的影响。
方法
将ARPE - 19细胞培养在微孔滤膜上形成汇合单层。将溴莫尼定添加到顶侧培养基中，并通过测量TER评估细胞的屏障功能。对一部分细胞在缺氧条件下进行处理，并将给予溴莫尼定后观察到的TER变化与在常氧条件下细胞中观察到的变化进行比较。
结果
培养四周后，ARPE细胞膜达到的峰值电阻为29.1±7.97Ω·cm²。常氧条件下处理的细胞，其TER随溴莫尼定处理而增加；然而，缺氧条件下处理的细胞在给予溴莫尼定后TER没有变化。
结论
溴莫尼定处理可使ARPE - 19细胞的屏障功能增强。要了解这种屏障功能变化的确切机制还需要进一步研究。
Zotero 插件