人芽囊原虫和脆弱双核阿米巴在符合肠易激综合征标准的患者中。

Blastocystis hominis and Dientamoeba fragilis in patients fulfilling irritable bowel syndrome criteria.

机构信息

Department of Medicine, Aga Khan University Hospital, Karachi, Pakistan.

出版信息

Parasitol Res. 2010 Aug;107(3):679-84. doi: 10.1007/s00436-010-1918-7. Epub 2010 Jun 8.

DOI:10.1007/s00436-010-1918-7

PMID:20532564

Abstract

Studies have suggested a possible role for Blastocystis hominis and Dientamoeba fragilis in the etiology of irritable bowel syndrome (IBS). We studied the prevalence of B. hominis and D. fragilis in patients with IBS-diarrhea (IBS-D). Three hundred and thirty patients were enrolled, 171 (52%) with IBS-D and 159 (48%) were controls, respectively. Stool microscopy, culture, and polymerase chain reaction (PCR) for B. hominis and D. fragilis were done. B. hominis was positive by stool microscopy in 49% (83/171) of IBS compared to 24% (27/159) in control (p < 0.001). B. hominis culture was positive in 53% (90/171) in IBS compared to 16% (25/159) in control (p < 0.001). B. hominis PCR was positive in 44% (75/171) in IBS compared to 21% (33/159) in control (p < 0.001). D. fragilis microscopy was positive in 3.5% (6/171) in IBS-D compared to 0.6% (1/159) in control (p = 0.123). D. fragilis culture was positive in 4% (7/171) in IBS compared to 1.3% (2/159) in control (p = 0.176). D. fragilis PCR was positive in 4% (6/171) in IBS-D compared to 0% (0/159) in control (p = 0.030). B. hominis is common, while D. fragilis was less prevalent in our patients with IBS-D. B. hominis and D. fragilis culture had a better yield compared to stool microscopy and PCR.

摘要

研究表明，人芽囊原虫和脆弱双核阿米巴可能在肠易激综合征（IBS）的病因中起作用。我们研究了人芽囊原虫和脆弱双核阿米巴在腹泻型肠易激综合征（IBS-D）患者中的流行情况。共纳入 330 例患者，其中 171 例（52%）为 IBS-D，159 例（48%）为对照组。进行了粪便显微镜检查、培养和聚合酶链反应（PCR）检测人芽囊原虫和脆弱双核阿米巴。与对照组（24%，27/159）相比，IBS 组（49%，83/171）的粪便显微镜检查人芽囊原虫阳性率更高（p<0.001）。IBS 组的人芽囊原虫培养阳性率为 53%（90/171），而对照组为 16%（25/159）（p<0.001）。IBS 组的人芽囊原虫 PCR 阳性率为 44%（75/171），而对照组为 21%（33/159）（p<0.001）。IBS-D 患者中，脆弱双核阿米巴镜检阳性率为 3.5%（6/171），对照组为 0.6%（1/159）（p=0.123）。IBS 组的脆弱双核阿米巴培养阳性率为 4%（7/171），对照组为 1.3%（2/159）（p=0.176）。IBS-D 患者中，脆弱双核阿米巴 PCR 阳性率为 4%（6/171），而对照组为 0%（0/159）（p=0.030）。我们的患者中，人芽囊原虫很常见，而脆弱双核阿米巴则较少见。与粪便显微镜检查和 PCR 相比，人芽囊原虫和脆弱双核阿米巴培养具有更好的检出率。