Universidade Estadual Paulista, Instituto de Biociências, Departmento de Parasitologia, Botucatu, São Paulo, Brazil.
Faculdades Integradas de Bauru, Bauru, São Paulo, Brazil.
Rev Inst Med Trop Sao Paulo. 2021 May 7;63:e39. doi: 10.1590/S1678-9946202163039. eCollection 2021.
In order to provide additional data on the prevalence and genetic diversity of Dientamoeba fragilis in human populations, we conducted a study in children from low-income communities in Sao Paulo State, Brazil. Fecal samples from daycare center attendees up to 6 years old (n=156) and staff members (n=18) were submitted to PCR and sequencing of D. fragilis as well as to microscopic examination for the presence of other intestinal parasites. All children assessed were asymptomatic and 10.3% (16/156) were positive for D. fragilis. No worker was found to be positive. An association between Dientamoeba and coinfection with other intestinal parasites was observed. Concerning the genetic diversity, 14 and only two isolates were genotype 1 and genotype 2, respectively. Our findings outline interesting aspects: (1) asymptomatic children as carriers of Dientamoeba in communities in which environmental conditions ensure parasite transmission and, (2) association between Dientamoeba infection in young children and coinfection with other enteric parasites, reinforcing its transmission via the fecal-oral route.
为了提供关于人体中脆弱双核阿米巴（Dientamoeba fragilis）流行率和遗传多样性的更多数据，我们在巴西圣保罗州的低收入社区的儿童中进行了一项研究。对日托中心的 6 岁以下儿童（n=156）和工作人员（n=18）的粪便样本进行了 PCR 和脆弱双核阿米巴的测序，以及对其他肠道寄生虫存在情况的显微镜检查。所有接受评估的儿童均无症状，10.3%（16/156）的儿童脆弱双核阿米巴检测呈阳性。未发现工作人员呈阳性。观察到脆弱双核阿米巴与其他肠道寄生虫合并感染之间存在关联。关于遗传多样性，分别有 14 个和仅 2 个分离株分别为基因型 1 和基因型 2。我们的研究结果概述了一些有趣的方面：（1）无症状儿童作为在确保寄生虫传播的环境条件下的社区中脆弱双核阿米巴的携带者，以及（2）幼儿中脆弱双核阿米巴感染与其他肠道寄生虫合并感染之间的关联，这加强了其通过粪-口途径传播。
