Department of Medicine, The Aga Khan University, Karachi, Pakistan.
Parasitol Res. 2010 Apr;106(5):1033-8. doi: 10.1007/s00436-010-1761-x. Epub 2010 Feb 23.
Blastocystis hominis is the most common intestinal parasite in humans. An extensive genetic variability has been described recently in B. hominis isolates. The aim of this study was to analyze genotypes of B. hominis isolates obtained from the healthy individuals and patients with irritable bowel syndrome-diarrhea (IBS-D). The patients with IBS-D were enrolled from gastroenterology outpatient department at the Aga Khan University Hospital. History and physical examination was done. Stool microscopy, culture, and polymerase chain reaction for B. hominis genotyping were carried out. The study included 158 patients with IBS-D, mean age 41 +/- 15, age range 16-83 years, and male/female ratio of 109:49. One hundred fifty-seven (49.8%) were taken as healthy control. The dominant B. hominis genotypes were genotype 1 in 87 (65%) and type 3 in 49 (37%). In IBS-D, genotype 1 was present in 75 (86%; P < 0.001) compared to 12 (14%) in controls while type 3 was present in 23 (47%) compared to 26 (53%) in controls (P < 0.001), respectively. Infection with single genotype of B. hominis was present in 70 (73%) with IBS-D and in 26 (27%) in control group while with multiple genotypes in 25 (64%) in IBS-D and 14 (36%) in control group (P = 0.30), respectively. Majority of our patients had typeable B. hominis infection. The genotype of B. hominis in IBS-D was type 1 while in control genotype 3 was predominant.
人芽囊原虫是人体内最常见的肠道寄生虫。最近，人芽囊原虫分离株的广泛遗传变异性已被描述。本研究旨在分析从健康个体和腹泻型肠易激综合征（IBS-D）患者中获得的人芽囊原虫分离株的基因型。IBS-D 患者从 Aga Khan 大学医院的胃肠病门诊招募。进行了病史和体格检查。进行了粪便显微镜检查、培养和人芽囊原虫基因分型聚合酶链反应。该研究包括 158 名 IBS-D 患者，平均年龄 41 ± 15 岁，年龄范围 16-83 岁，男女比例为 109:49。157 名（49.8%）被视为健康对照。主要的人芽囊原虫基因型为基因型 1（87 例，占 65%）和基因型 3（49 例，占 37%）。在 IBS-D 中，基因型 1 存在于 75 例（86%；P < 0.001），而对照中存在 12 例（14%），而基因型 3 存在于 23 例（47%），而对照中存在 26 例（53%）（P < 0.001）。IBS-D 中 70 例（73%）存在单一基因型人芽囊原虫感染，对照组 26 例（27%）存在单一基因型人芽囊原虫感染，而 IBS-D 中 25 例（64%）存在多重基因型人芽囊原虫感染，对照组 14 例（36%）存在多重基因型人芽囊原虫感染（P = 0.30）。我们的大多数患者都有人芽囊原虫感染。IBS-D 中的人芽囊原虫基因型为 1 型，而对照组中的基因型为 3 型。
