Mumcuoğlu Ipek, Coşkun Feride Alaca, Aksu Neriman, Pürnak Tuğrul, Güngör Ciğdem
Ankara Numune Eğitim ve Araştırma Hastanesi, Tıbbi Mikrobiyoloji Kliniği, Ankara, Turkey.
Turkiye Parazitol Derg. 2013;37(2):73-7. doi: 10.5152/tpd.2013.19.
We investigated the prevalence of Dientamoeba fragilis and Blastocystis spp. in IBS patients and evaluated whether there was a possible link between IBS and these parasitic infections.
Stool specimens collected from 55 IBS patients, 80 patients with gastroenteritis as control group 1 (CG-1) and 50 healthy volunteers as control group 2 (CG-2) were included the study. Samples were examined by direct microscopy, trichrome staining and culture methods.
While there was no significant difference in the prevalence of Blastocystis spp. between IBS patients and CG-1 (p > 0.05), a significant difference was found between IBS and CG-2 (p < 0.05). Patients with IBS were found to have five or more Blastocystis spp. per field than control groups. After eradication, all symptoms were cured in four patients, there were only constipation problems left in eleven patients and there were no changes in clinical findings in three patients. D. fragilis was not found in any of the samples.
The reason we did not find any D. fragilis may be due to the low infection rate in the region. However, significantly having five or more Blastocystis spp. per field (X40) in IBS patients and regression of IBS symptoms after treatment in most of the patients suggested a possible link between IBS and Blastocystis spp.
我们调查了肠易激综合征（IBS）患者中脆弱双核阿米巴和芽囊原虫的感染率，并评估了IBS与这些寄生虫感染之间是否存在可能的联系。
本研究纳入了从55例IBS患者、80例作为对照组1（CG-1）的肠胃炎患者以及50例作为对照组2（CG-2）的健康志愿者收集的粪便标本。样本通过直接显微镜检查、三色染色和培养方法进行检测。
虽然IBS患者与CG-1之间芽囊原虫的感染率没有显著差异（p>0.05），但IBS与CG-2之间存在显著差异（p<0.05）。发现IBS患者每视野中芽囊原虫的数量比对照组多五个或更多。根除后，四名患者的所有症状均治愈，十一名患者仅遗留便秘问题，三名患者的临床检查结果无变化。在任何样本中均未发现脆弱双核阿米巴。
未发现任何脆弱双核阿米巴的原因可能是该地区的感染率较低。然而，IBS患者每视野（×40）中显著有五个或更多的芽囊原虫，并且大多数患者治疗后IBS症状消退，提示IBS与芽囊原虫之间可能存在联系。
