Department of Biotechnology, Thapar University, Patiala, 147 004, Punjab, India.
J Basic Microbiol. 2013 Sep;53(9):752-7. doi: 10.1002/jobm.201200163. Epub 2012 Sep 7.
2,4,5-trichlorophenol (2,4,5-TCP) mineralizing bacteria were isolated from the secondary sludge of pulp and paper industry. These isolates used 2,4,5-TCP as a source of carbon and energy and were capable of degrading this compound, as indicated by stoichimetric release of chloride and biomass formation. Based on 16S rRNA sequence analysis, these bacteria were identified as Kocuria sp. (CL2), Bacillus pumillus (CL5), Pseudomonas stutzeri (CL7). HPLC analysis revealed that these isolates were able to degrade 2,4,5-TCP at higher concentrations (600 mg/l or 3.0 mM). A consortia of these isolates completely removed 2,4,5-TCP from the sludge obtained from pulp and paper mill within 2 weeks when supplemented at a rate of 100 mg l(-1) . Bacterial consortium also significantly reduced absorbable organic halogen (AOX) and extractable organic halogen (EOX) by 61% and 63%, respectively from the sludge. These isolates have high potential to remove 2,4,5-TCP and may be used for remediation of pulp paper mill waste containing 2,4,5-TCP.
从制浆造纸工业的二级污泥中分离出了 2,4,5-三氯苯酚（2,4,5-TCP）矿化细菌。这些分离物将 2,4,5-TCP 用作碳源和能源，并且能够降解该化合物，这表现为化学计量释放氯化物和生物量形成。基于 16S rRNA 序列分析，这些细菌被鉴定为节杆菌属（CL2）、短小芽孢杆菌（CL5）和施氏假单胞菌（CL7）。HPLC 分析表明，这些分离物能够在较高浓度（600mg/l 或 3.0mM）下降解 2,4,5-TCP。当以 100mg l(-1) 的速率添加时，这些分离物的细菌群落能够在 2 周内从制浆造纸厂污泥中完全去除 2,4,5-TCP。细菌群落还使污泥中的可吸收有机卤素（AOX）和可萃取有机卤素（EOX）分别显著减少了 61%和 63%。这些分离物具有去除 2,4,5-TCP 的巨大潜力，可用于修复含有 2,4,5-TCP 的制浆造纸厂废物。
