Ito Koji, Takagi Kazuhiro, Matsushima Yoshitaka, Iwasaki Akio, Tanaka Naoto, Kanesaki Yu, Martin-Laurent Martin-Laurent Fabrice Fabrice, Igimi Shizunobu
Department of Agricultural Chemistry, Tokyo University of Agriculture.
Hazardous Chemicals Division, Institute for Agro-Environmental Sciences, NARO.
J Pestic Sci. 2018 May 20;43(2):124-131. doi: 10.1584/jpestics.D17-089.
While genes are well known in Gram-negative bacteria to code for the enzymes responsible for pentachlorophenol (CHClO; PCP) degradation, little is known about PCP-degrading genes in Gram-positive bacteria. Here we describe a novel gene operon possibly responsible for catalyzing the degradation of PCP in the Gram-positive bacterium sp. strain PD653, which is capable of mineralizing hexachlorobenzene (CCl; HCB) PCP. Transcriptome analysis based on RNA-Seq revealed overexpressed genes in strain PD653 following exposure to HCB. Based on annotation, three open reading frames (ORFs) were selected as biodegrading enzyme candidates. Recombinant cells expressing candidate genes degraded approximately 9.4 µmol L PCP in 2 hr. Therefore, we designated these genes as . Interestingly, PCP-degrading activity was recorded when was coexpressed with or , and the function of HcbB3 was expected to be similar to chlorophenol 4-monooxygenase (TftD).
虽然在革兰氏阴性细菌中，基因编码负责五氯苯酚（CHClO；PCP）降解的酶已广为人知，但对于革兰氏阳性细菌中PCP降解基因却知之甚少。在此，我们描述了一个新的基因操纵子，它可能负责催化革兰氏阳性细菌sp. 菌株PD653中PCP的降解，该菌株能够将六氯苯（CCl；HCB）矿化。基于RNA测序的转录组分析揭示了菌株PD653在暴露于HCB后过表达的基因。基于注释，选择了三个开放阅读框（ORF）作为生物降解酶候选基因。表达候选基因的重组细胞在2小时内降解了约9.4 μmol·L的PCP。因此，我们将这些基因命名为 。有趣的是，当 与 或 共表达时记录到了PCP降解活性，并且预计HcbB3的功能与氯酚4-单加氧酶（TftD）相似。
