深入了解光合细菌荚膜红细菌的蛋白质组。
A glimpse into the proteome of phototrophic bacterium Rhodobacter capsulatus.
作者信息
Onder Ozlem, Aygun-Sunar Semra, Selamoglu Nur, Daldal Fevzi
机构信息
Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA.
出版信息
Adv Exp Med Biol. 2010;675:179-209. doi: 10.1007/978-1-4419-1528-3_11.
A first glimpse into the proteome of Rhodobacter capsulatus revealed more than 450 (with over 210 cytoplasmic and 185 extracytoplasmic known as well as 55 unknown) proteins that are identified with high degree of confidence using nLC-MS/MS analyses. The accumulated data provide a solid platform for ongoing efforts to establish the proteome of this species and the cellular locations of its constituents. They also indicate that at least 40 of the identified proteins, which were annotated in genome databases as unknown hypothetical proteins, correspond to predicted translation products that are indeed present in cells under the growth conditions used in this work. In addition, matching the identification labels of the proteins reported between the two available R. capsulatus genome databases (ERGO-light with RRCxxxxx and NT05 with NT05RCxxxx numbers) indicated that 11 such proteins are listed only in the latter database.
对荚膜红细菌蛋白质组的初步研究揭示，通过nLC-MS/MS分析，可以高度确信地鉴定出450多种蛋白质（其中有210多种存在于细胞质中，185种存在于细胞质外，还有55种未知）。积累的数据为正在进行的建立该物种蛋白质组及其成分细胞定位的工作提供了坚实的平台。这些数据还表明，在基因组数据库中注释为未知假设蛋白的已鉴定蛋白质中，至少有40种对应于在本研究使用的生长条件下确实存在于细胞中的预测翻译产物。此外，比对两个可用的荚膜红细菌基因组数据库（带有RRCxxxxx编号的ERGO-light和带有NT05RCxxxx编号的NT05）中报告的蛋白质识别标签发现，有11种此类蛋白质仅在后一个数据库中列出。
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