Deutsches Institut für Kautschuktechnologie e.V., Eupener Strasse 33, 30519 Hannover, Germany.
J Mater Sci Mater Med. 2010 Jul;21(7):2115-24. doi: 10.1007/s10856-010-4072-y. Epub 2010 Jun 9.
Fiber scaffolds of bioactive polysialic acid have been prepared via electrospinning for peripheral nerve regeneration. The diameter, morphology and alignment of fibers in scaffolds were adjusted by variation of electrospinning parameters, which are decisive for the cell-scaffold interaction. Due to the high water solubility of polysialic acid (poly-alpha-2,8-N-acetylneuraminic acid) a photoactive derivative (poly-alpha-2,8-N-pentenoylneuraminic acid) was used to obtain stable fiber scaffolds in water by photochemical crosslinking. At the optimized fiber scaffolds good cell viability and directed cell proliferation along the fibers was achieved by cell tests with immortalized Schwann cells.
生物活性聚唾液酸的纤维支架已通过静电纺丝制备用于周围神经再生。支架中纤维的直径、形态和排列通过改变静电纺丝参数进行调整，这对细胞-支架相互作用至关重要。由于聚唾液酸（聚α-2,8-N-乙酰神经氨酸）的水溶性很高，因此使用光活性衍生物（聚α-2,8-N-戊烯酰神经氨酸）通过光化学交联在水中获得稳定的纤维支架。在优化的纤维支架中，通过永生化雪旺细胞的细胞试验实现了良好的细胞活力和沿纤维的定向细胞增殖。
