Arkhipova S S, Raginov I S, Mukhitov A R, Chelyshev Y A
Microscopy Laboratory, Kazan Institute of Biochemistry and Biophysics, Russian Academy of Sciences, Kazan, Russia.
Neurosci Behav Physiol. 2010 Jul;40(6):609-14. doi: 10.1007/s11055-010-9303-7. Epub 2010 Jun 9.
Sciatic nerve crushing, transection, and ligation models were used in rats to study the reactions of and changes in the numbers of satellite cells (SC) in spinal dorsal root ganglia in the lumbar segment. Nerve transection was followed by the appearance of neurons surrounded by two layers of SC. The thickness of SC processes and the areas of contacts with neurons increased as a result of invaginations into neuron perikarya. After nerve ligation, SC and their processes were located around parts of large and intermediate neurons in several tightly appressed layers; the area of contact between SC and neuron perikarya showed increased development of invaginations such that lamellar structures appeared in the SC cytoplasm, along with contacts with SC processes surrounding neighboring neurons. The greatest increases in SC numbers were seen after ligation of the nerve. Transection was followed by increases in the numbers of small and intermediate neurons surrounded by vimentin-positive SC. The number of large neurons surrounded by these cells decreased. At all time points following ligation of the nerve, all neurons in the study ganglia were surrounded by vimentin-positive SC. Post-traumatic changes in structure and numbers differed in SC associated with sensory neurons of individual size populations and depended on the type of trauma applied to efferent conductors.
在大鼠中采用坐骨神经挤压、横断和结扎模型，以研究腰段脊髓背根神经节中卫星细胞（SC）的反应及数量变化。神经横断后，出现了被两层卫星细胞包围的神经元。卫星细胞突起的厚度以及与神经元的接触面积因向神经元胞体内陷而增加。神经结扎后，卫星细胞及其突起位于几层紧密贴附的大神经元和中间神经元周围；卫星细胞与神经元胞体之间的接触面积显示内陷增加，从而在卫星细胞胞质中出现板层结构，同时与周围神经元周围的卫星细胞突起也有接触。神经结扎后卫星细胞数量增加最多。横断后，被波形蛋白阳性卫星细胞包围的小神经元和中间神经元数量增加。被这些细胞包围的大神经元数量减少。在神经结扎后的所有时间点，研究神经节中的所有神经元均被波形蛋白阳性卫星细胞包围。与不同大小群体的感觉神经元相关的卫星细胞在创伤后的结构和数量变化有所不同，且取决于施加于传出传导通路的创伤类型。
