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人体皮肤上潜在指纹和DNA的提取

Recovery of latent fingerprints and DNA on human skin.

作者信息

Färber Doris, Seul Andrea, Weisser Hans-Joachim, Bohnert Michael

机构信息

German Federal Criminal Police, Wiesbaden, Germany.

出版信息

J Forensic Sci. 2010 Nov;55(6):1457-61. doi: 10.1111/j.1556-4029.2010.01476.x.

Abstract

The project "Latent Fingerprints and DNA on Human Skin" was the first systematic research in Europe dealing with detection of fingerprints and DNA left by offenders on the skin of corpses. One thousand samples gave results that allow general statements on the materials and methods used. The tests were carried out according to a uniform trial structure. Fingerprints were deposited by natural donors on corpses. The latent fingerprints were treated with magnetic powder or black fingerprint powder. Afterward, they were lifted with silicone casting material (Isomark(®)) or gelatine foil. All lifts were swabbed to recover DNA. It was possible to visualize comparable and identifiable fingerprints on the skin of corpses (16%). In the same categories, magnetic powder (18.4%) yielded better results than black fingerprint powder (13.6%). The number of comparable and identifiable fingerprints decreased on the lifts (12.7%). Isomark(®) (14.9%) was the better lifting material in comparison with gelatine foil (10.1%). In one-third of the samples, DNA could be extracted from the powdered and lifted latents. Black fingerprint powder delivered the better result with a rate of 2.2% for full DNA profiles and profiles useful for exclusion in comparison with 1.8% for the magnetic powder traces. Isomark(®) (3.1%) yielded better results than gelatine foil (0.6%).

摘要

“人体皮肤上的潜在指纹与DNA”项目是欧洲首个针对罪犯留在尸体皮肤上的指纹和DNA检测展开的系统性研究。一千个样本得出的结果有助于对所使用的材料和方法进行总体陈述。测试按照统一的试验结构进行。指纹由自然捐献者留在尸体上。潜在指纹用磁粉或黑色指纹粉处理。之后,用硅酮铸造材料(Isomark(®))或明胶箔进行提取。对所有提取物进行擦拭以获取DNA。有可能在尸体皮肤上显现出可比较且可识别的指纹(16%)。在同一类别中,磁粉(18.4%)的效果优于黑色指纹粉(13.6%)。提取物上可比较且可识别的指纹数量有所减少(12.7%)。与明胶箔(10.1%)相比,Isomark(®)(14.9%)是更好的提取材料。在三分之一的样本中,可以从粉末状和提取物中的潜在指纹中提取DNA。黑色指纹粉的效果更好,完整DNA图谱和可用于排除的图谱比例为2.2%,而磁粉痕迹的这一比例为1.8%。Isomark(®)(3.1%)的效果优于明胶箔(0.6%)。

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