Gelderman M P, Yazer M H, Jia Y, Wood F, Alayash A I, Vostal J G
Laboratory of Cellular Hematology, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Rockville, Maryland, USA.
Transfus Med. 2010 Oct;20(5):341-5. doi: 10.1111/j.1365-3148.2010.01016.x.
To contribute to the understanding of the biochemical changes associated with the RBC storage lesion.
To investigate changes in O(2) equilibrium and on/off kinetic rates during routine cold storage.
As RBCs are stored between 1 and 6°C numerous biochemical changes occur within the RBCs, including changes in the properties of the haemoglobin itself. This study serially analysed for the first time the O(2) equilibrium and on/off kinetic rates across the RBC membrane during routine storage.
METHODS/MATERIALS: The oxygen binding (k(on) ) and offloading (k(off) ) constants were measured in fresh RBCs and then in AS-5-preserved RBCs at weekly intervals, along with oxygen equilibrium curves (OECs), 2,3-Diphosphoglycerate (2,3-DPG), p50 and the Hill number (n).
The k(on) increased slightly as the 2,3-DPG and p50 decreased during storage, whereas the k(off) remained largely unchanged. The OECs demonstrated the expected increase in O(2) affinity, whereas the Hill number was unchanged during storage.
In spite of the biochemical, structural and functional changes associated with the storage of RBCs, their in vitro interactions with oxygen were largely preserved through 42 days of storage.
促进对与红细胞储存损伤相关的生化变化的理解。
研究常规冷藏期间氧气平衡以及结合/解离动力学速率的变化。
红细胞在1至6°C储存时，红细胞内会发生许多生化变化，包括血红蛋白自身性质的改变。本研究首次对常规储存期间红细胞膜上的氧气平衡以及结合/解离动力学速率进行了连续分析。
方法/材料：在新鲜红细胞中测量氧气结合常数（k(on)）和解离常数（k(off)），然后在AS-5保存的红细胞中每周测量一次，同时测量氧气平衡曲线（OECs）、2,3-二磷酸甘油酸（2,3-DPG）、p50和希尔系数（n）。
储存期间，随着2,3-DPG和p50降低，k(on)略有增加，而k(off)基本保持不变。氧气平衡曲线显示氧气亲和力如预期那样增加，而储存期间希尔系数不变。
尽管红细胞储存会伴随生化、结构和功能变化，但在42天的储存期内，其与氧气的体外相互作用基本得以保留。
