Department of Anesthesiology, Duke University Medical Center, Durham, North Carolina 27710, USA.
Transfusion. 2012 Nov;52(11):2459-64. doi: 10.1111/j.1537-2995.2012.03615.x. Epub 2012 Mar 27.
Stored red blood cells (RBCs) accumulate biochemical and biophysical changes. Maximum storage duration is based on acceptable in vitro characteristics and 24-hour survival, but not RBC function. Relatively little is known about the impact of RBC storage duration on oxygenation and the microcirculation.
Eight healthy subjects donated a double RBC apheresis, which were prestorage leukoreduced and processed in AS-3. Subjects were transfused 1 unit of RBCs at 7 and 42 days after blood collection. Measurements of percentage of tissue oxygenation in the thenar eminence muscle (StO2) and brain (SctO2) were recorded with Food and Drug Administration-cleared noninvasive devices. Sublingual microvascular blood flow (microcirculatory flow index [MFI]) was quantified before and after RBC transfusion using a video microscope. Raw electronic data for all measurements were analyzed by a blinded observer at a core laboratory.
The only pre- versus posttransfusion change observed in measurements of SctO2, StO2, or MFI was a very small increase in SctO2, from 70.4 to 71.8 (means, p=0.032) at 7 days. There was no significant difference in the amount of pre-post change at 7 days versus 42 days for any of the measures.
Transfusion of 1 unit of 42-day-stored RBCs to healthy subjects has no overt detrimental effect on tissue oxygenation or the microcirculation assessed by clinically available monitors.
储存的红细胞 (RBC) 会积累生化和生物物理变化。最大储存时间基于可接受的体外特性和 24 小时存活率，而不是 RBC 功能。相对而言，人们对 RBC 储存时间对氧合和微循环的影响知之甚少。
8 名健康受试者捐献了两次 RBC 单采，这些 RBC 经白细胞减少处理并在 AS-3 中进行处理。受试者在采血后 7 天和 42 天各输注 1 单位 RBC。使用经食品和药物管理局批准的非侵入性设备记录大鱼际肌（StO2）和大脑（SctO2）的组织氧饱和度百分比。在 RBC 输注前后使用视频显微镜定量舌下微血管血流（微循环血流指数 [MFI]）。所有测量的原始电子数据均由核心实验室的盲法观察者进行分析。
在 SctO2、StO2 或 MFI 的测量中，唯一观察到的预输注与输注后变化是 SctO2 非常小的增加，从 70.4 增加到 71.8（平均值，p=0.032），输注时间为 7 天。在 7 天与 42 天之间，任何测量的预输注后变化量均无显着差异。
向健康受试者输注 1 单位储存 42 天的 RBC 对组织氧合或通过临床可用监测器评估的微循环没有明显的不良影响。
