Center for Biomaterials, University of Connecticut Health Center, Farmington, CT 06030, USA.
J Biomater Sci Polym Ed. 2010;21(10):1371-87. doi: 10.1163/092050609X12517190417830. Epub 2010 Jun 8.
A simple method is reported for fabricating polystyrene disk inserts coated with biomimetic carbonated hydroxyapatite (cHA) to be used for culturing osteoprogenitor cells or other stem cells. Roughened disks cut from tissue-culture polystyrene (TCPS) were coated in simulated body fluid with 5 x normal physiologic ionic concentrations (SBFx5) by a 2-step, 2-day method. The coatings were rigorously characterized by various methods and assessed in cell culture. An adherent, nearly 10 mm thick, relatively uniform layer of single-phase cHA was formed in two days. MC3T3-E1 and mouse calvaria-derived osteoprogenitor cells (pCOBs) were cultured on the cHA for various time points. Despite less initial attachment of both cell types to the cHA, proliferation rates on cHA were similar to that on TCPS. Two-fold greater cell attachment (P < 0.05) of the MC3T3-E1 cells was observed relative to the pCOBs, on both the TCPS and the cHA. Importantly, the coatings were relatively smooth, without the extensive agglomerates observed in other studies and remained adherent and morphologically unchanged after 21 days of culture. This technique can be used to rapidly produce high-quality cHA-coated TCPS disks for cell-culture studies.
报告了一种简单的方法来制备涂有仿生碳酸化羟基磷灰石（cHA）的聚苯乙烯盘插入物，用于培养成骨细胞前体细胞或其他干细胞。通过两步法和两天法，用 5 x 正常生理离子浓度（SBFx5）在组织培养聚苯乙烯（TCPS）上切割的粗糙盘进行涂层。通过各种方法对涂层进行了严格的表征，并在细胞培养中进行了评估。在两天内形成了一层附着的、近 10 毫米厚、相对均匀的单相 cHA 层。将 MC3T3-E1 和鼠颅骨来源的成骨细胞前体细胞（pCOBs）在 cHA 上培养不同的时间点。尽管两种细胞类型对 cHA 的初始附着较少，但 cHA 上的增殖速率与 TCPS 上的相似。与 pCOBs 相比，MC3T3-E1 细胞对 TCPS 和 cHA 的附着增加了两倍（P < 0.05）。重要的是，涂层相对光滑，没有在其他研究中观察到的广泛团聚体，并且在培养 21 天后仍然附着并且形态没有变化。这种技术可用于快速制备用于细胞培养研究的高质量 cHA 涂层 TCPS 盘。
