利用 HCP5 单核苷酸多态性预测阿巴卡韦过敏反应：与 HLA-B*5701 的相关性。
Use of the HCP5 single nucleotide polymorphism to predict hypersensitivity reactions to abacavir: correlation with HLA-B*5701.
机构信息
Pharmacology and Pharmacogenetic Unit, Hospital Carlos III, Madrid, Spain.
出版信息
J Antimicrob Chemother. 2010 Aug;65(8):1567-9. doi: 10.1093/jac/dkq204. Epub 2010 Jun 9.
OBJECTIVES
To study the correlation between the HLA-B*5701 allele and the single nucleotide polymorphism in HCP5 (rs2395029).
PATIENTS AND METHODS
All HIV patients naive for abacavir seen at our institution between September 2007 and December 2008 were prospectively screened for HLA-B*5701. HCP5 rs2395029 genotyping was carried out by allelic discrimination using the TaqMan 5'-nuclease assay. High-resolution HLA class I typing was undertaken using sequence-specific primers.
RESULTS
A total of 245 HIV patients were included in the study. A good correlation between HLA-B*5701 and HCP5 was observed (negative and positive predictive values of 100% and 93%, respectively).
CONCLUSIONS
The use of HCP5 rs2395029 testing could be as useful as HLA-B*5701 typing to prevent the abacavir hypersensitivity reaction. Given that HCP5 testing is cheaper, less time-consuming and easier to perform than HLA typing, it may confidently replace the latter in clinical settings.
目的
研究 HLA-B*5701 等位基因与 HCP5(rs2395029)单核苷酸多态性之间的相关性。
患者与方法
本机构于 2007 年 9 月至 2008 年 12 月期间所有初次使用阿巴卡韦的 HIV 患者均前瞻性地筛查 HLA-B*5701。采用 TaqMan 5’-核酸酶检测方法进行 HCP5(rs2395029)等位基因特异性检测。采用序列特异性引物进行高分辨率 HLA I 类基因分型。
结果
共有 245 例 HIV 患者纳入本研究。HLA-B*5701 与 HCP5 之间存在良好的相关性（阴性和阳性预测值均为 100%和 93%）。
结论
与 HLA-B*5701 基因分型相比，使用 HCP5(rs2395029)检测有助于预防阿巴卡韦超敏反应。鉴于 HCP5 检测比 HLA 基因分型更便宜、耗时更短、操作更简单，它可以在临床环境中替代后者。
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