Pharmacology and Pharmacogenetic Unit, Hospital Carlos III, Madrid, Spain.
J Antimicrob Chemother. 2010 Aug;65(8):1567-9. doi: 10.1093/jac/dkq204. Epub 2010 Jun 9.
To study the correlation between the HLA-B*5701 allele and the single nucleotide polymorphism in HCP5 (rs2395029).
All HIV patients naive for abacavir seen at our institution between September 2007 and December 2008 were prospectively screened for HLA-B*5701. HCP5 rs2395029 genotyping was carried out by allelic discrimination using the TaqMan 5'-nuclease assay. High-resolution HLA class I typing was undertaken using sequence-specific primers.
A total of 245 HIV patients were included in the study. A good correlation between HLA-B*5701 and HCP5 was observed (negative and positive predictive values of 100% and 93%, respectively).
The use of HCP5 rs2395029 testing could be as useful as HLA-B*5701 typing to prevent the abacavir hypersensitivity reaction. Given that HCP5 testing is cheaper, less time-consuming and easier to perform than HLA typing, it may confidently replace the latter in clinical settings.
研究 HLA-B*5701 等位基因与 HCP5(rs2395029)单核苷酸多态性之间的相关性。
本机构于 2007 年 9 月至 2008 年 12 月期间所有初次使用阿巴卡韦的 HIV 患者均前瞻性地筛查 HLA-B*5701。采用 TaqMan 5’-核酸酶检测方法进行 HCP5(rs2395029)等位基因特异性检测。采用序列特异性引物进行高分辨率 HLA I 类基因分型。
共有 245 例 HIV 患者纳入本研究。HLA-B*5701 与 HCP5 之间存在良好的相关性（阴性和阳性预测值均为 100%和 93%）。
与 HLA-B*5701 基因分型相比，使用 HCP5(rs2395029)检测有助于预防阿巴卡韦超敏反应。鉴于 HCP5 检测比 HLA 基因分型更便宜、耗时更短、操作更简单，它可以在临床环境中替代后者。
