Miki S, Tanouchi Y, Otani T, Yamaguchi K, Mimura Y
Department of Ophthalmology, Tokushima University School of Medicine, Japan.
Nippon Ganka Gakkai Zasshi. 1991 Feb;95(2):152-7.
A study was made of endogenous uveitis in experimental disseminated intravascular coagulation (DIC) in rabbits. Endotoxin was injected intravenously twice with a 24-hour interval. The time courses of the following were examined: 1) aqueous flare using a laser flare-cell meter 2) the number of leukocytes in the peripheral blood 3) the tumor necrosis factor (TNF) activity in the serum and 4) histopathological changes in the eye, lung, liver and kidney. Aqueous flare increased at 1 hour and was maximal at 6 hours, accompanied by a rapid increase in TNF activity at 1 hour following the first endotoxin administration. The number of leukocytes decreased to 963 +/- 266 cells/mm3 at 1.5 hours with subsequent leukocytosis within 12 hours. After the second injection of endotoxin, the aqueous flare peaked in 30 minutes and was twice as high as the first peak. Leukocyte number and TNF activity showed the same behavior. However, TNF activity was 20% that of the first peak. Histopathological examination indicated fibrin formation in the small vessels of systemic organs within 3 hours following the second administration of endotoxin. Endotoxin induced uveitis was induced in experimental DIC, and leukocytes and TNF activity may thus perform important roles.
对兔实验性弥散性血管内凝血（DIC）中的内源性葡萄膜炎进行了研究。静脉注射内毒素，间隔24小时注射两次。检查了以下各项的时间进程：1）使用激光散射细胞仪测量房水闪光；2）外周血白细胞数量；3）血清中肿瘤坏死因子（TNF）活性；4）眼、肺、肝和肾的组织病理学变化。房水闪光在1小时时增加，6小时时达到最大值，同时在首次给予内毒素后1小时TNF活性迅速增加。白细胞数量在1.5小时时降至963±266个细胞/mm³，随后在12小时内出现白细胞增多。第二次注射内毒素后，房水闪光在30分钟时达到峰值，是第一个峰值的两倍。白细胞数量和TNF活性表现出相同的变化。然而，TNF活性是第一个峰值的20%。组织病理学检查表明，在第二次给予内毒素后3小时内，全身器官的小血管中有纤维蛋白形成。内毒素诱导的葡萄膜炎在实验性DIC中被诱发，因此白细胞和TNF活性可能起重要作用。
