Staibano Stefania, Mascolo Massimo, Di Benedetto Maria, Vecchione Maria Luisa, Ilardi Gennaro, Di Lorenzo Giuseppe, Autorino Riccardo, Salerno Vincenzo, Morena Antonella, Rocco Alba, Turco Maria Caterina, Morelli Emilio
Department of Biomorphological and Functional Sciences, Pathology Section, University Federico II of Naples, School of Medicine, Via S. Pansini, 5, 80131, Naples, Italy.
Tumour Biol. 2010 Oct;31(5):461-9. doi: 10.1007/s13277-010-0055-3. Epub 2010 Jun 10.
Despite the progressive increase of early diagnosis, a subset of prostate cancers show a metastasizing and lethal course, not always predictable upon the traditional prognostic parameters. The object of this study was to investigate the role of the survival co-chaperone protein BAG3 as a new prognostic marker for prostate cancer. BAG3 was detected by immunohistochemistry in 55 specimens of surgically removed prostate carcinomas and in 15 surgical specimens of non-neoplastic prostate tissues. Results were compared with clinic-pathological data and outcome of patients and statistically evaluated. BAG3 resulted expressed in all the cases: Non-neoplastic prostate tissue showed a cytoplasmatic staining with apical reinforcement, a finding which appears consistent with the reported connection of the protein with the membrane focal cell-adhesion complexes. In prostate carcinomas, BAG3 showed a progressive decrease of the expression level from well- to low-differentiated carcinoma, coupled with the loss of polarisation of the signal in metastasizing cases. These results indicate that BAG3 intra-cytoplasmic delocalisation is a specific feature of cancer versus non-neoplastic prostate and a candidate new marker for prediction of prostate cancer invasiveness and behaviour.
尽管早期诊断在不断进步，但仍有一部分前列腺癌呈现出转移和致死的病程，这并不总是能通过传统的预后参数预测出来。本研究的目的是探讨生存共伴侣蛋白BAG3作为前列腺癌新的预后标志物的作用。通过免疫组织化学方法，在55例手术切除的前列腺癌标本和15例非肿瘤性前列腺组织手术标本中检测BAG3。将结果与患者的临床病理数据和预后进行比较，并进行统计学评估。结果显示，所有病例中均检测到BAG3表达：非肿瘤性前列腺组织显示细胞质染色且顶端增强，这一发现与该蛋白与膜局部细胞粘附复合物的报道联系一致。在前列腺癌中，BAG3表达水平从高分化癌到低分化癌逐渐降低，并且在转移病例中信号的极性丧失。这些结果表明，BAG3在细胞质中的异位是癌症与非肿瘤性前列腺的一个特定特征，并且是预测前列腺癌侵袭性和行为的一个候选新标志物。
