Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Central 4, 1-1-1 Higashi, Tsukuba, Ibaraki, Japan.
Cytoskeleton (Hoboken). 2010 Aug;67(8):496-503. doi: 10.1002/cm.20460.
Previously, we reported that a nanoneedle of 200 nm diameter manipulated by an atomic force microscope apparatus could be inserted into a living cell. The insertion probabilities varied according to cell type. However, the nanoneedle was never successfully inserted into artificial liposomes. In the current study, we found that the stress fibers and actin filaments comprising the plasmalemmal undercoat are important, determining factors as to whether a nanoneedle can be successfully inserted into a cell. Depolymerization of microtubules increased both the number of stress fibers and insertion efficiency in NRK cells. These results indicate that the insertion efficiency of a nanoneedle (200 nm in diameter) into a cell with a smaller actin meshwork in its plasmalemmal undercoat is enhanced and the formation of stress fibers obviously contributes to this incremental enhancement. These facts are not only important as technical information to improve the efficiency of cell manipulation but also as observations of the mechanical properties of the native cell cortex.
此前，我们曾报道过，利用原子力显微镜设备操纵直径为 200nm 的纳米针可以插入活细胞。插入概率因细胞类型而异。然而，纳米针从未成功插入人工脂质体中。在本研究中，我们发现构成质膜下皮层的应力纤维和肌动蛋白丝是决定纳米针能否成功插入细胞的重要因素。微管的解聚增加了 NRK 细胞中的应力纤维数量和插入效率。这些结果表明，插入效率纳米针（直径 200nm）进入具有较小的肌动蛋白网格的质膜下皮层的细胞增强，并且应力纤维的形成明显有助于这种增量增强。这些事实不仅作为提高细胞操作效率的技术信息很重要，而且作为对天然细胞皮质机械性能的观察也很重要。
