Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University , Toyonaka, Osaka , Japan.
Department of Life and Environment Engineering, Faculty of Environmental Engineering, The University of Kitakyushu , Kitakyushu, Fukuoka , Japan.
PeerJ. 2015 Jul 30;3:e1131. doi: 10.7717/peerj.1131. eCollection 2015.
The mechanical features of individual animal cells have been regarded as indicators of cell type and state. Previously, we investigated the surface mechanics of cancer and normal stromal cells in adherent and suspended states using atomic force microscopy. Cancer cells possessed specific mechanical and actin cytoskeleton features that were distinct from normal stromal cells in adherent and suspended states. In this paper, we report the unique mechanical and actin cytoskeletal features of human embryonic kidney HEK293 cells. Unlike normal stromal and cancer cells, the surface stiffness of adherent HEK293 cells was very low, but increased after cell detachment from the culture surface. Induced actin filament depolymerization revealed that the actin cytoskeleton was the underlying source of the stiffness in suspended HEK293 cells. The exclusive mechanical response of HEK293 cells to perturbation of the actin cytoskeleton resembled that of adherent cancer cells and suspended normal stromal cells. Thus, with respect to their special cell-surface mechanical features, HEK293 cells could be categorized into a new class distinct from normal stromal and cancer cells.
个体动物细胞的力学特性被视为细胞类型和状态的指标。此前，我们使用原子力显微镜研究了贴壁和悬浮状态下的癌细胞和正常基质细胞的表面力学特性。癌细胞具有独特的力学特性和肌动蛋白细胞骨架特征，与贴壁和悬浮状态下的正常基质细胞明显不同。在本文中，我们报告了人胚肾 HEK293 细胞的独特力学特性和肌动蛋白细胞骨架特征。与正常基质细胞和癌细胞不同，贴壁 HEK293 细胞的表面硬度非常低，但在从培养表面脱离后增加。诱导肌动蛋白丝解聚表明，肌动蛋白细胞骨架是悬浮 HEK293 细胞硬度的潜在来源。HEK293 细胞对肌动蛋白细胞骨架扰动的独特力学响应类似于贴壁癌细胞和悬浮正常基质细胞。因此，就其特殊的细胞表面力学特征而言，HEK293 细胞可以归入与正常基质细胞和癌细胞不同的新类别。
