Zhao Wei, Wen Hai-xia, Zheng Hui-li
Department of Physiology, Harbin Medical University, Harbin 150086.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2010 Mar;30(3):286-90.
To investigate the effect and mechanism of Zuoguiyin (ZGY, a Chinese medical remedy for regulating qi and nourishing yin) on expression of ovarian follicle-stimulating hormone receptor (FSHR) in rats during peri-menopausal period (PMP).
PMP rats were administered with low (13.78 g/kg), middle (20.67 g/kg) and high (31.00 g/kg) dose ZGY respectively by gastrogavage for eight weeks. FSHR mRNA and protein expressions were detected by RT-PCR and immunohistochemistry respectively. Besides, granulosa cells, collected from pregnant mare serum treated nonage rats, were incubated, and the level of FSHR mRNA expression in the cultured cells were detected after various disposals.
(1) Ovarian levels of FSHR mRNA and protein expressions in PMP rats were significantly lower than those in young rats (P<0.01), but were up-regulated significantly by ZGY treatment (P<0.01). (2) As compared with the control, FSHR mRNA expression increased in cultured granulosa cells after treated by ZGY extract; the increasing effect of ZGY was enhanced by combined treatment with Forskolin and attenuated by Genistein (a tyrosine protein kinase inhibitor).
ZGY could improve the ovarian functions during PMP by up-regulating the expression of FSHR and raise the ovarian responsibility to FSH, which may be possibly realized by activating cAMP-protein kinase and tyrosine protein kinase signal pathway.
探讨左归饮（一种中医理气滋阴方剂）对围绝经期（PMP）大鼠卵巢卵泡刺激素受体（FSHR）表达的影响及机制。
将PMP大鼠分别以低（13.78 g/kg）、中（20.67 g/kg）、高（31.00 g/kg）剂量左归饮灌胃给药8周。分别采用逆转录-聚合酶链反应（RT-PCR）和免疫组化法检测FSHR mRNA和蛋白表达。此外，收集孕马血清处理的幼年大鼠的颗粒细胞进行培养，经不同处理后检测培养细胞中FSHR mRNA表达水平。
（1）PMP大鼠卵巢FSHR mRNA和蛋白表达水平显著低于幼年大鼠（P<0.01），但左归饮治疗后显著上调（P<0.01）。（2）与对照组相比，左归饮提取物处理后培养的颗粒细胞中FSHR mRNA表达增加；福斯高林联合处理增强了左归饮的增加作用，而染料木黄酮（一种酪氨酸蛋白激酶抑制剂）则减弱了该作用。
左归饮可通过上调FSHR表达改善PMP大鼠的卵巢功能，提高卵巢对FSH的反应性，这可能是通过激活环磷酸腺苷-蛋白激酶和酪氨酸蛋白激酶信号通路实现的。
