Zhou Jue, Qu Fan, Jin Yue, Yang Dong-Xia
College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, Zhejiang, China.
Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
Afr J Tradit Complement Altern Med. 2014 Aug 23;11(5):1-7. doi: 10.4314/ajtcam.v11i5.1. eCollection 2014.
Bisphenol-A (BPA) is one of the widespread industrial compounds, which has adverse effects on animal and human health. The study was aimed to explore the effects of Crassostrea gigas extracts (CGE) in alleviating ovarian functional disorders of female rats with exposure to BPA and the underlying possible mechanism.
Eighteen four-week-old female Sprague-Dawley (SD) rats were randomly divided into BPA group (50mg/kg BPA), BPA+CGE group (50mg/kg BPA+50mg/kg CGE), and control group (equivalent dosage of vehicle) with 6 rats in each group. After a 6-week treatment ended, the serum levels of estradiol (E2), follicle stimulating hormone (FSH), luteinizing hormone (LH) were measured by using commercial standard assay kits. The expression levels of FSH receptor (FSHR) in the rat ovarian tissues were respectively detected by immunohistochemistry and Real-time PCR.
CGE treatment markedly increased E2 levels and decreased FSH levels in the serum (P<0.05), however, the alterations of serum LH levels were not significant (P>0.05). The protein and mRNA expression levels of FSHR were the lowest in the ovaries of control rats and the highest in BPA rats (P<0.05). CGE treatment markedly decreased the expression levels of FSHR in the ovarian tissues (P<0.05).
Crassostrea gigas successfully alleviates ovarian functional disorders of female rats with exposure to BPA partly through decreasing FSHR expression levels in the ovarian tissues.
双酚A（BPA）是一种广泛存在的工业化合物，对动物和人类健康具有不良影响。本研究旨在探讨太平洋牡蛎提取物（CGE）对暴露于BPA的雌性大鼠卵巢功能障碍的缓解作用及其潜在的可能机制。
将18只4周龄的雌性Sprague-Dawley（SD）大鼠随机分为BPA组（50mg/kg BPA）、BPA+CGE组（50mg/kg BPA+50mg/kg CGE）和对照组（等量赋形剂），每组6只。6周治疗结束后，使用商业标准检测试剂盒测定血清雌二醇（E2）、卵泡刺激素（FSH）、黄体生成素（LH）水平。采用免疫组织化学和实时荧光定量PCR分别检测大鼠卵巢组织中FSH受体（FSHR）的表达水平。
CGE治疗显著提高了血清中E2水平并降低了FSH水平（P<0.05），然而，血清LH水平的变化不显著（P>0.05）。FSHR的蛋白和mRNA表达水平在对照组大鼠卵巢中最低，在BPA组大鼠卵巢中最高（P<0.05）。CGE治疗显著降低了卵巢组织中FSHR的表达水平（P<0.05）。
太平洋牡蛎成功缓解了暴露于BPA的雌性大鼠的卵巢功能障碍，部分原因是通过降低卵巢组织中FSHR的表达水平。
