Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut, USA.
Ann N Y Acad Sci. 2010 Jun;1197:76-84. doi: 10.1111/j.1749-6632.2010.05191.x.
Investigations into the possible roles of human HSPB1 in aging have focused on its role as a molecular chaperone protecting partially folded or unfolded proteins, particularly during oxidative stress. A thorough analysis of potential roles of HSPB1 in aging cells has been hampered by a limited knowledge of its functions in living cells. Most studies have employed cell-free extracts and purified proteins. For example, HSPB1 is known to bind actin in vitro, and this observation led to the hypothesis that HSPB1 regulates actin filament dynamics. In the study summarized herein, the role of HSPB1 in regulating actin filament dynamics was further investigated by using cultured human cells. These results show that HSPB1 and actin form a complex in vivo and that HSPB1 is important for cell motility. A model for HSPB1 as a regulator of actin filament dynamics is presented, and evidence from the literature on cytoskeletal alterations in aging cells is discussed.
对人类 HSPB1 在衰老过程中可能作用的研究集中在其作为分子伴侣保护部分折叠或未折叠蛋白质的作用上，尤其是在氧化应激期间。由于对 HSPB1 在活细胞中的功能了解有限，因此对 HSPB1 在衰老细胞中的潜在作用的全面分析受到了阻碍。大多数研究都使用无细胞提取物和纯化蛋白。例如，HSPB1 已知在体外与肌动蛋白结合，这一观察结果导致了 HSPB1 调节肌动蛋白丝动力学的假说。在本文总结的研究中，通过使用培养的人细胞进一步研究了 HSPB1 在调节肌动蛋白丝动力学中的作用。这些结果表明，HSPB1 和肌动蛋白在体内形成复合物，并且 HSPB1 对于细胞迁移是重要的。提出了 HSPB1 作为肌动蛋白丝动力学调节剂的模型，并讨论了文献中关于衰老细胞中细胞骨架改变的证据。
